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2 protocols using anti gfap g3893

1

Molecular Profiling of Neurodegeneration

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Dulbecco's modified Eagle's medium medium (#12100046), fetal bovine serum (#26140079), penicillin/streptomycin (#15140122), OptiMEM (#31985062), Lipofectamine (#11668030), PBS (#14190), chemiluminescence kit (#32106), and Imperial protein stain (#24615) were purchased from Thermo Fisher. Th-S (T1892) and Th-T (T3516) were purchased from Sigma. Anti-ITM2B (sc-374362) was purchased from Santa Cruz; anti-ApoE (66830) from ProteinTech; anti-C1Q (ab182451) from Abcam; anti-FCRL4 (PA5-87813) from Invitrogen; anti-GFAP (G3893) from Sigma; and anti-Myc (sc-40) and anti-Tubulin (sc-23948) from Santa Cruz. Anti-ADan (ab 1700) polyclonal antibody has been reported previously (10 (link)).
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2

Immunohistochemical Staining of Neural Markers

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The rabbit polyclonal antibody against recombinant human galectin-1 (anti-rhGAL-1) we used in this study has been described previously [24] . A mouse monoclonal antibody against glial fibrillary acidic protein (anti-GFAP) (G3893, 1:500), a marker for astrocytes, was obtained from Sigma-Aldrich Japan KK (Tokyo, Japan). A rat monoclonal antibody against CD11b (Ab8878, 1:500), a marker for microglia, was obtained from Abcam KK (Tokyo, Japan). A mouse monoclonal antibody against neurofilament 200 (N5389, 1:500) and a mouse monoclonal antibody against β-actin (A5316, 1:10,000) were obtained from Sigma-Aldrich Japan KK (Tokyo, Japan). Alexa Fluor-labeled secondary antibodies were obtained from Invitrogen Japan (Tokyo, Japan).
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