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5 protocols using hydrochloric acid 37

1

Magnolol and Honokiol Extraction Protocol

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Magnolol and honokiol (98% purity) were acquired from New Natural Biotechnology (Shanghai, China). Phosphotungstic acid solution, sodium phosphate monobasic and potassium phosphate monobasic were purchased from Sigma-Aldrich (Madrid, Spain). N-octanol, acetonitrile, ethyl alcohol, and potassium chloride were obtained from VWR chemicals (Barcelona, Spain). Hydrochloric acid 37% and sodium hydroxide were purchased from Scharlab (Barcelona, Spain). Hydrogen peroxide 30% and boric acid were obtained from Merck (Barcelona, Spain). Phospholipon 90 G was provided by Lipoid GmbH (Ludwigshafen, Germany).
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2

Quantitative Analysis of Methylprednisolone

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Methyl Prednisolone reference standard (MP), Methyl Prednisolone hemisuccinate reference standard (MPHS), and Methylprednisolone sodium succinate working standard (MPSS) was supplied by UP pharma (Assuit, Egypt). Acetonitrile HPLC-grade, disodium hydrogen phosphate, sodium dihydrogen phosphate, glacial acetic acid 99%, hydrochloric acid 37%, sodium hydroxide, and Hydrogen peroxide 30% (Scharlau, Spain). Water for injection (WFI) was used in the analysis and passed through a 0.45 μm nylon membrane filter before use. Phosphate solution (1) was prepared by weighing 1.6 g of disodium hydrogen phosphate in 1000 mL of WFI. Phosphate solution (2) was prepared by weighing about 0.3 g of sodium dihydrogen phosphate in 1000 mL of WFI.
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3

Quantitative Analysis of Cefazolin Sodium

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Cfz sodium working standard was supplied as a gift sample from UP Pharma (Assuit, Egypt). ACNHPLC-grade, Potassium dihydrogen phosphate, Hydrochloric acid 37%, Sodium hydroxide, and Hydrogen peroxide 30% (Scharlau, Spain). Water for injection (WFI) was used in the analysis and passed through a 0.45 μm nylon membrane filter before use. Phosphate solution was prepared by weighing about 5.82 g of Potassium dihydrogen phosphate in 1000 mL of WFI.
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4

Antimicrobial Chitosan-based Formulations

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Low molecular weight (LMW) chitosan (75 -85% deacetylation degree), glacial acetic acid and all aldehydes used in this work were provided by Sigma-Aldrich (Barcelona-Spain). Citric acid monohydrate, and disodium phosphate to carry out buffer medium at pH 7 and pH 4 was also purchased from Sigma-Aldrich (Barcelona-Spain). Hydrochloric acid 37%, ethanol 96%, di-phosphorus pentoxide and granulated sodium hydroxide of synthesis grade were supplied by Scharlab (Barcelona, Spain). Milli-Q water was obtained by Milli-Q Plus purification system (Millipore, Molsheim, France). Escherichia coli (CECT 434) and Listeria innocua (CECT 910) was supplied by the Spanish Type Culture Collection (CECT, Valencia, Spain) . The yeast Sacharomyces cerevisae var. ellipsoideys (NCYC 2959) was supplied by National Collection of Yeasts Cultures (NCYC). All culture medium employed were supplied by Scharlab (Barcelona, Spain).
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5

DPPH Radical Scavenging and ACE Inhibition

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2, 2′-diphenyl-1-picrylhydrazyl (DPPH) and ACE enzyme (from rabbit lung) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Abz-Gly-p-nitro-phe-pro-OH trifluoroacetate salt used as substrate was purchased from Bachem AG. (Bubendorf, Switzerland). Microbial culture media were purchased from Sharlau (Barcelona, Spain).
Acetonitrile, hydrochloric acid 37%, and trifluoroacetic acid (TFA) were of HPLC grade (Scharlau). All other chemicals were of analytical grade (Scharlau).
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