I1784

We used the following antibodies: anti-GFP (A11122, Life technologies), anti-GAPDH (sc-25778, Santa Cruz), anti-Histone (07–449, Millipore), anti-Flag (F3165, Sigma), anti-Actin (MAB1501R, Chemicon), anti-Hey1 (anti-HRT1, sc-16424, Santa Cruz), anti-p53 (SAPU, [53 (link)]), anti-Ptc (sc-6149, Santa Cruz), anti-TrkC (AF1404, R&D), anti-MDM2 (VMA00406, BioRad), anti-COBRA1 (F7E4, GeneTex), anti-BAX (sc-526, Tebu Bio), anti-BAK (G-23, Santa Cruz), anti-NT-3 (sc-547, Santa Cruz), and anticleaved PARP (9541T, Cell Signaling). The following antibodies were used for proximity ligation assays (DuoLink): anti-GFP (TP401, Biolabs), anti-Flag (F3165, Sigma), anti-Hey1 (anti-HRT1, sc-16424, Santa Cruz), anti-p53 (sc-126, Santa Cruz), and anti-importins (I1784, Sigma Aldrich).

The following antibodies were used for immunofluorescence (IF) and western blotting (WB): rat monoclonal anti-importin-α1 (1:100 for IF, 1:1000 for WB; 2G7; ab22534; Abcam), rat polyclonal anti-importin-α1 (1:100 for IF, 1:1000 for WB; I9658; Sigma-Aldrich), mouse monoclonal anti-importin-α (1:100 for IF, 1:1000 for WB; I1784; Sigma-Aldrich), mouse monoclonal anti-importin-β (1:100 for IF, 1:1000 for WB; 3E9; ab2811; Abcam), rabbit polyclonal anti-importin-β (raised against GST-tagged full-length importin-β and used at 1:1000 for WB), anti-histone H3 phospho-S10 (1:2000 for WB; 05-1336; Sigma-Aldrich), anti-α-tubulin (1:100 for IF, 1:2000 for WB; T9026; Sigma-Aldrich), anti-GFP (1:2000 for WB; sc-9996; Santa Cruz Biotechnology), anti-KIFC1 (in-house polyclonal, raised against his-tagged 887-end of KIFC1 and used at 1:100 for IF, 1:1000 for WB), rabbit polyclonal anti-TPX2 (1:100 for IF, 1:1000 for WB; 12245; Cell Signaling Technology), anti-NuMA (1:1000 for WB; ab109262; Abcam), anti-Ran (1:5000 for WB; 610340; BD Biosciences) and anti-Myc (1:1000 for WB; MABE282; Sigma-Aldrich). Kinase inhibitors were used at the following concentrations: 10 µM purvalanol A(Sigma-Aldrich), 9 µM Ro-3306 (Sigma-Aldrich), 0.1 µM BI2536 (Axon Medchem), 2 µM ZM447439(Selleck), 0.5 μM LY294009 (Selleck).