Biological grade sterile dmso

The stock cultures of Gram-negative bacteria, Escherichia coli ATCC 8739, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 9027, Gram-positive bacteria as Staphylococcus aureus ATCC 25923, Streptococcus faecalis ATCC 8043, Listeria monocytogenes ATCC 7644, and the fungus Candida albicans ATCC 10231 were obtained from Microlab, Institute of Research and Technology, Vellore, Tamilnadu, India. The microbiological growth media, Mueller-Hinton agar, and Mueller-Hinton broth were obtained from Oxoid, ThermoFisher Scientific (MA, United States). Positive control antibiotic 6.0 mm discs as Chloramphenicol (C30), Gentamycin (CN10), Ceftriaxone (CEC30), and Cefoxitin (FOX30) were purchased from Oxoid, ThermoFisher Scientific (MA, United States), while biological grade sterile DMSO from ThermoFisher Scientific (MA, United States).

All chemicals, reagents, and Nunc Micro-well™ plates were purchased from Sigma Aldrich (Milan, Italy and St. louis, MO, United states) except otherwise stated. Oxygen radical absorbance capacity was recorded using FLUOstar OPTIMA (Franka Ganske, BMG LABTECH, Offenburg, Germany), while ELX 808microplate reader (BioTek, Italy) was adopted to measure the absorbance’s in the other assays. The extracted volatile constituents by hydro distillation and supercritical fluid were analyzed on Shimadzu GC/MS-QP2010 linked to a quadrupole mass spectrometer (Shimadzu Corporation, Kyoto, Japan) supplemented with Rtx-5MS column (30 m × 0.25 mm i.d. × 0.25-μm film thickness, Restek, United States). For the antimicrobial assays, the stock cultures of Gram-positive bacteria, Staphylococcus aureus (ATCC 25923), Streptococcus pyogenes (ATCC 12344), Clostridium perfringens (ATCC 13124), and the Gram-negative Pseudomonas aeruginosa (ATCC 9027) were supplied from Microlab, Institute of Research and Technology (Vellore, Tamilnadu, India). Mueller–Hinton agar and broth, biological grade sterile DMSO, chloramphenicol (C), and gentamycin (CN) 6.0 mm discs (positive control antibiotic) were purchased from Oxoid, Thermo Fisher Scientific (MA, United States).