Cells were washed with ice-cold PBS three times and treated with 1–5mM disuccinimidyl suberate (DSS, A39267, Thermo Scientific, Waltham, MA) for 30 min at room temperature. The reaction was stopped by adding the quenching solution (1M Tris, PH 7.5) to the final concentration of 20mM for 15 min. Then, cell lysates were used for WB as described above.
A39267
Manufactured by Thermo Fisher Scientific
The A39267 is a laboratory instrument designed for conducting various analyses and experiments. It is a versatile piece of equipment that can be used in a wide range of scientific applications. The core function of the A39267 is to perform accurate measurements and data collection, enabling researchers and scientists to gather reliable information for their studies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using a39267
1
Crosslinking Cells with DSS
2
Protein Crosslinking and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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1x10 6 cells were precipitated and washed once with 1xPBS. The cell pellet was resuspended in 500 µL of 1xPBS. Cell suspensions were treated with either DMSO (control) or 1mM DSS (A39267, Thermo Fisher) and incubated for 30min at RT. Then, the cells were quenched with 50mM Tris-HCl pH:8.0 by incubating for 15min at RT. Finally, the cells were centrifuged, and the cell pellets were boiled in 50 µL of 2x SDS-Laemmli Sample Buffer for 10 min at 95 0 C.
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