Bacillus subtilis CICC10012 (B. subtilis), Salmonella enterica WX29 (S. enterica), Staphyloccocus aureus CICC10201 (S. aureus), Bacillus cereus ATCC11778 (B. cereus), Escherichia coli CICC10899 (E. coli), Pseudomonas brenneri CICC10271 (P. brenneri) were purchased from the China Center of Industrial Culture Collection (Beijing, China) and were cultivated in Luria Bertani (LB) broth with aeration at 37 °C. L. rhamnosus YT, preserved by the key laboratory of dairy biotechnology and safety control of Jiangsu province, was isolated from the feces of Bama longevity, Guangxi province, China. L. rhamnosus YT was inoculated in dMRS broth at 37 °C in static condition. The biomass was determined by viable counts [39 (link)].
Bacillus subtilis cicc10012
Manufactured by China Center of Industrial Culture Collection
Sourced in China
Bacillus subtilis CICC10012 is a strain of the bacterium Bacillus subtilis, maintained in the China Center of Industrial Culture Collection. It is a laboratory strain used for research and experimental purposes.
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Lab products found in correlation
3 protocols using bacillus subtilis cicc10012
1
Microbial Cultivation and Biomass Determination
2
Screening Lactobacillus Strains' Antimicrobial Activity
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A total of 40 Lactobacillus strains isolated from traditional fermented dairy samples, fermented vegetables, and human intestine tracts were cultured in MRS medium at 37°C for 24 h. Lactobacillus rhamnosus GG (LGG) obtained from the Shanghai Bioresources Collection Center was cultured under the same condition and used as the reference strain.
The indicator strains Bacillus subtilis CICC10012, Escherichia coli CICC10899, and Salmonella WX29 obtained from China Center of Industrial Culture Collection (CICC) were cultured in Luria‐Bertani (LB) medium at 37°C for 12 h.
The indicator strains Bacillus subtilis CICC10012, Escherichia coli CICC10899, and Salmonella WX29 obtained from China Center of Industrial Culture Collection (CICC) were cultured in Luria‐Bertani (LB) medium at 37°C for 12 h.
3
Probiotic Strains Inhibition Assay
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The LAB strains of Lactobacillus rhamnosus grx19, L. acidophilus ss, L. plantarum s7, L. casei g9, Bifidobacterium animalis BLC and Streptococcus thermophiles 90–57 were isolated from the gut of subjects from Bama longevity, Guangxi province, China, in 2013. The strains were separately cultured in skim milk broth for 12 h at 37 °C. The bacteria and supernatant were separated by centrifugation. The harvested bacteria were suspended by saline to 1 × 108 CFU mL−1. The supernatant was filtered by 0.22 μm Millipore filtration for the assay.
Escherichia coli CICC10899, Salmonella enterica WX29, Staphylococcus aureus CICC10201, Pseudomonas brenneri CICC10271, Clostridium difficile CICC22951, Bacillus subtilis CICC10012 were bought from China Center of Industrial Culture Collection (Beijing, China). All the strains were cultivated in Luria–Bertani broth at 37 °C except the Clostridium difficile CICC22951 cultivated in Reinforced Clostridium Medium broth. The bacteria were harvested after incubation for12 h and suspended to 1 × 107 CFU mL−1 by saline.
Escherichia coli CICC10899, Salmonella enterica WX29, Staphylococcus aureus CICC10201, Pseudomonas brenneri CICC10271, Clostridium difficile CICC22951, Bacillus subtilis CICC10012 were bought from China Center of Industrial Culture Collection (Beijing, China). All the strains were cultivated in Luria–Bertani broth at 37 °C except the Clostridium difficile CICC22951 cultivated in Reinforced Clostridium Medium broth. The bacteria were harvested after incubation for12 h and suspended to 1 × 107 CFU mL−1 by saline.
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