Adipose tissue was immediately collected after terminations and fixed in 10% PBS-buffered formalin for 24 h. Following paraffin embedding, the tissue sections were stained with a primary antibody against Endomucin (1:200, R&D systems. Minneapolis, MN) or Mac-2 (1 mg/ml, 1:1,000; Tebu-Bio, France) followed by donkey α-goat Alexa Fluor 647-conjugated secondary antibodies (1.5 μg/mL, Jackson Immuno-Research Laboratories, West Grove, PA). Nuclei were stained with DAPI (1 μg/mL, Vector Laboratories, Burlingame, CA). Images were acquired with Carl Zeiss upright Apotome Axio Imager Z1/ZEN2 Core Imaging software.
Endomucin
Manufactured by R&D Systems
Sourced in China
Endomucin is a laboratory reagent produced by R&D Systems. It is a glycoprotein that is expressed on the surface of endothelial cells. Endomucin plays a role in cell-cell interactions and cell adhesion.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 647 conjugated secondary antibody, by Jackson ImmunoResearch (2 mentions)
Mac 2, by Tebu-Bio (2 mentions)
Upright apotome axio imager z1 zen2 core imaging software, by Zeiss (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions)
Phospho stat3 tyr705, by Cell Signaling Technology (1 mentions)
Ly6g 1a8, by BD (1 mentions)
3 protocols using endomucin
1
Adipose Tissue Immunofluorescence Staining
2
Immunostaining of Heart Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The hearts were fixed overnight with 4% PFA and embedded in paraffin. Ly-6G (1A8) (BD Pharmingen), phospho-STAT3 (Tyr705) (Cell Signaling Technology) or endomucin (R&D systems) immunostaining was conducted on 3-μm tissue sections using the Streptavidin-Peroxidase Kit (Zhongshan jinqiao, Beijing, China), followed by staining with hematoxylin. The slides were dehydrated in ethanol twice for 3 min each and were subsequently mounted for light microscopy.
3
Adipose Tissue Immunofluorescence Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adipose tissue was immediately collected after terminations and fixed in 10% PBS-buffered formalin for 24 h. Following paraffin embedding, the tissue sections were stained with a primary antibody against Endomucin (1:200, R&D systems. Minneapolis, MN) or Mac-2 (1 mg/ml, 1:1,000; Tebu-Bio, France) followed by donkey α-goat Alexa Fluor 647-conjugated secondary antibodies (1.5 μg/mL, Jackson Immuno-Research Laboratories, West Grove, PA). Nuclei were stained with DAPI (1 μg/mL, Vector Laboratories, Burlingame, CA). Images were acquired with Carl Zeiss upright Apotome Axio Imager Z1/ZEN2 Core Imaging software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!