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Tfap2a

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TFAP2A is a transcription factor that regulates gene expression. It plays a role in the development and differentiation of various cell types.

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Lab products found in correlation

Cleaved caspase 3, by Abcam (1 mentions) Tcf21, by Abcam (1 mentions) Caspase 3, by Proteintech (1 mentions) Gapdh, by Proteintech (1 mentions) Bcl xl, by Proteintech (1 mentions) P akt, by Proteintech (1 mentions)

2 protocols using tfap2a

1

Chromatin Immunoprecipitation (ChIP) Assay

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The ChIP assays were conducted according manufacturer’s protocol (The SimpleChIP® Plus Enzymatic Chromatin IP Kit: #9005, CST). Briefly, cells were fixed with formaldehyde, and the chromatin was fragmented by enzymatic digestion and sonication. Then precleared chromatin was immunoprecipitated overnight with specific antibodies (TFAP2A: 13019–3-AP, Proteintech, Wuhan, China; Normal Rabbit IgG: #2729, CST; Histone H3 (D2B12) XP® Rabbit mAb: #4620, CST). The enrichment of specific DNA fragments was analyzed by qPCR. The primers used are listed and explained in Supplementary Table 3.
Xiong Y., Feng Y., Zhao J., Lei J., Qiao T., Zhou Y., Lu Q., Jiang T., Jia L, & Han Y. (2021). TFAP2A potentiates lung adenocarcinoma metastasis by a novel miR-16 family/TFAP2A/PSG9/TGF-β signaling pathway. Cell Death & Disease, 12(4), 352.
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2

Western Blotting Protein Extraction Protocol

Cited 1 time
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The protein extraction method and detailed steps of western blotting were adapted from a previous study [23 (link)]. The following antibodies were used: TCF21 and cleaved caspase 3 from Abcam (Britain) and p-AKT, AKT, caspase 3, BCL-xL, TFAP2A, and GAPDH from Proteintech (Wuhan, China).
Zhou X., Shen K., Cao S., Li P., Xiao J., Dong J., Cheng Q., Hu L., Xu Z, & Yang L. (2024). Polymorphism rs2327430 in TCF21 predicts the risk and prognosis of gastric cancer by affecting the binding between TFAP2A and TCF21. Cancer Cell International, 24, 159.
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