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Bmi1creer

Manufactured by Jackson ImmunoResearch
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Bmi1CreER is a genetic tool used for lineage tracing and cell type-specific gene manipulation in mice. It expresses a tamoxifen-inducible Cre recombinase under the control of the Bmi1 promoter, allowing for temporal control of Cre activity and targeted genetic modifications in Bmi1-expressing cell populations.

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Lab products found in correlation

R26 tdtomato, by Jackson ImmunoResearch (1 mentions) Nog mice, by Taconic Biosciences (1 mentions) Lgr5 egfp ires creer, by Jackson ImmunoResearch (1 mentions)

3 protocols using bmi1creer

1

Generating Mouse HNSCC Model with 4-NQO

Cited 2 times
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Bmi1CreER;R26tdTomato mice were generated by crossmating Bmi1CreER (Jackson Laboratory, JAX:010531) with R26tdTomato (Jackson Laboratory, JAX:007908) as described before (Chen et al., 2017 (link)). NOG mice were purchased from Taconic. Mice were housed under standard conditions in the animal facility of UCLA. All procedures were performed based on the UCLA Animal Research Committee-approved protocols. The induction of mouse HNSCC with 4-NQO was performed as described before (Jia et al., 2020 (link)).
Wang C., Li Y., Jia L., Kim J.K., Li J., Deng P., Zhang W., Krebsbach P.H, & Wang C.Y. (2021). CD276 expression enables squamous cell carcinoma stem cells to evade immune surveillance. Cell stem cell, 28(9), 1597-1613.e7.
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2

Genetic Tracing of Adult Stem Cells

Cited 1 time
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Lgr5−EGFP-IRES-CreER (JAX mice strain 008875), Bmi1-CreER (JAX strain 010531), and R26-CAG-LSL-tdTomato (JAX mice strain 007914) mice were obtained from the Jackson Laboratory. Hopx-CreER (JAX strain 017606) mice were generated at the University of Pennsylvania. Mice were maintained on a C57/BL6N background. Axin2-CreERT2-tdTomato mice were generated by insertion of a CreERT2/tdTomato fusion cDNA downstream of the first ATG of the mouse Axin2 gene using homologous recombination in mouse ESCs, described in detail in (Choi et al., 2013 (link)). For CreERT2 induction experiments, mice received 1–3 daily intraperitoneal injections of 100 μl Tam (10 mg/ml in corn oil; Sigma; T5648). All mouse protocols were approved by the Institutional Animal Care and Use Committee at the University of Pennsylvania under protocol 803415 to Dr. Lengner.
Li N., Yousefi M., Nakauka-Ddamba A., Tobias J.W., Jensen S.T., Morrisey E.E, & Lengner C.J. (2016). Heterogeneity in readouts of canonical Wnt pathway activity within intestinal crypts. Developmental dynamics : an official publication of the American Association of Anatomists, 245(8), 822-833.
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3

Generation of Lineage-Tracing Mouse Models

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Bmi1CreER (JAX: 010531) and RosatdTomato (JAX: 007909) mouse strains were crossbred to generate Bmi1CreER; RosatdTomato mice. K14CreER (NM-KI-190024) and Mycflox/flox (NM-CKO-210184) mouse strains were crossbred to generate K14CreER; Mycflox/flox. Bmi1CreER and RosatdTomato mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). K14CreER and Mycflox/flox were purchased from Shanghai Model Organisms Center, Inc. (Shanghai, China). The NOD-SCID mice, and BALB/c-nude female mice were purchased from Beijing Sibeifu Biotechnology Co. Ltd (Beijing, China). All the above mice were housed under specific-pathogen-free (SPF) conditions. All animal studies were performed in compliance with the regulations and the Peking University institutional animal care guidelines.
Liu S., Qin Z., Mao Y., Wang N., Zhang W., Wang Y., Chen Y., Jia L, & Peng X. (2024). Pharmacological inhibition of MYC to mitigate chemoresistance in preclinical models of squamous cell carcinoma. Theranostics, 14(2), 622-639.
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