Nadph content assay kit

Manufactured by Solarbio

Sourced in China

The NADPH content assay kit is a laboratory tool designed to quantify the levels of nicotinamide adenine dinucleotide phosphate (NADPH) in biological samples. It provides a straightforward method for measuring NADPH concentrations accurately.

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Lab products found in correlation

Elisa assay kit, by Beyotime (1 mentions) Atp content assay kit, by Solarbio (1 mentions)

2 protocols using nadph content assay kit

Glucose Metabolism Assays in CRC Cells

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RKO and HCT116 cells were transfected with shRNA expression vectors or overexpression vectors. Glucose level in CRC cells was determined with a Glucose content assay kit. Lactate production was measured with a lactate assay kit.
G6PD activity was assessed with a
G6PD assay kit. The assay kits mentioned above were purchased from Cominbio (Suzhou, China). Intracellular NADPH level was assessed with an NADPH content assay kit (Solarbio, Beijing, China). To assess
G6PD activity in CRC samples, tissues were homogenized, and enzyme activity was assessed with the
G6PD assay kit and calculated relative to that of the control group. The total protein amount was measured using the ELISA assay kit (Beyotime, Shanghai, China).

Wu F., Zhang W., Wei H., Ma H., Leng G, & Zhang Y. (2023). lncRNA ELFN1-AS1 promotes proliferation, migration and invasion and suppresses apoptosis in colorectal cancer cells by enhancing G6PD activity : lncRNA ELFN1-AS1 promotes the progression of colorectal cancer. Acta Biochimica et Biophysica Sinica, 55(4), 649-660.

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Measurement of L-arginine, Glucose, Cell Growth, Nitrite, NADPH, ATP, NADH

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The concentration of L-arginine was measured using a Sykam S-433D amino acid analyzer (Sykam Co., Ltd., Germany) (Zhang et al., 2018 (link)). Glucose concentrations were determined enzymatically by a bioanalyzer (SBA-40E, Shandong, China). Cell growth was determined by measuring the OD562 (1 OD562 = 0.375 g L^–1 dry cell weight) with a spectrophotometer (BG-XM496, Beijing, China). Nitrite concentrations were determined using Nitrite Content Assay Kit (Solarbio, Beijing, China). NADPH concentrations were determined using NADPH Content Assay Kit (Solarbio, Beijing, China). ATP concentrations were determined using ATP Content Assay Kit (Solarbio, Beijing, China). NADH concentrations were determined using NADH Content Assay Kit (Beyotime, Shanghai, China). All experiments were performed in triplicates, and data were represented as the mean ± standard deviation.

Huang M., Zhu L., Feng L., Zhan L., Zhao Y, & Chen X. (2022). Reforming Nitrate Metabolism for Enhancing L-Arginine Production in Corynebacterium crenatum Under Oxygen Limitation. Frontiers in Microbiology, 13, 834311.

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