Tris 2 carboxyethyl phosphine hydrochloride

Manufactured by GoldBio

Tris(2-carboxyethyl)phosphine hydrochloride is a reducing agent commonly used in biochemical applications. It is a water-soluble compound that helps maintain a reducing environment in various experimental settings.

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Lab products found in correlation

Isopropyl β d 1 thiogalactopyranoside (iptg), by GoldBio (2 mentions) Dithiothreitol (dtt), by GoldBio (2 mentions) Sodium dithionite, by Merck Group (1 mentions) Ni nitrilotriacetic acid nta resin, by Qiagen (1 mentions) N 2 hydroxyethylpiperazine n 2 ethane sulfonic acid hepes, by Thermo Fisher Scientific (1 mentions) S adenosylhomocysteine sah, by Merck Group (1 mentions) Kanamycin, by GoldBio (1 mentions) Ampicillin, by GoldBio (1 mentions) Arabinose, by GoldBio (1 mentions) L arabinose, by Merck Group (1 mentions) Ampicillin, by Thermo Fisher Scientific (1 mentions) Chloramphenicol, by Merck Group (1 mentions) Guanidine hydrochloride, by Merck Group (1 mentions) Ristocetin, by MP Biomedicals (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

Spelling variants of this product

Tris(2-carboxyethyl)phosphine (3 citations) Tris (2‐carboxyethyl) phosphine hydrochloride (TCEP) (3 citations)

3 protocols using tris 2 carboxyethyl phosphine hydrochloride

Purity and Synthesis of Cofactors

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N-(2-Hydroxyethyl)-piperazine-N′-(2-ethanesulfonic acid) (HEPES) was purchased
from Fisher Scientific. Imidazole was purchased from J. T. Baker Chemical
Co. Potassium chloride and glycerol were purchased from EMD Chemicals.
β-Mercaptoethanol (BME), sodium dithionite, sodium sulfide,
5′-deoxyadenosine (5′-dAH), and S-adenosylhomocysteine
(SAH) were purchased from MilliporeSigma. Kanamycin, ampicillin, dithiothreitol
(DTT), arabinose, isopropyl β-d-1-thiogalactopyranoside
(IPTG) and tris(2-carboxyethyl)phosphine hydrochloride (TCEP) were
purchased from Gold Biotechnology. Ni-nitrilotriacetic acid (NTA)
resin was acquired from Qiagen. SAM was synthesized and purified as
described previously.^{45 (link)} DNA isolation kits
were purchased from Macherey-Nagel (Dueren, Germany). All other chemicals
and materials were of the highest grade available and were from MilliporeSigma.

Neti S.S., Sil D., Warui D.M., Esakova O.A., Solinski A.E., Serrano D.A., Krebs C, & Booker S.J. (2022). Characterization of LipS1 and LipS2 from Thermococcus kodakarensis: Proteins Annotated as Biotin Synthases, which Together Catalyze Formation of the Lipoyl Cofactor. ACS Bio & Med Chem Au, 2(5), 509-520.

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Bacterial Growth Media Protocols

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Tryptic Soy Broth (TSB, Becton, Dickinson) was used as rich medium for
Staphylococcus aureus strains. Nutrient broth (NB) (Difco)
supplemented with NaCl (85 mM) was used as rich medium for Salmonella
enterica strains. No-carbon Essential (NCE) minimal medium (Berkowitz et al., 1968 (link))
supplemented with trace minerals (Balch &
Wolfe, 1976), L-methionine (100 μM), and magnesium sulfate (1
mM) was used to grow S. enterica strains. When present in the
medium, acetate was at 10 mM. All strains were grown at 37 ºC with
shaking (180 rpm). When added to the medium, antibiotics were present at the
following concentrations: ampicillin (Fisher Scientific), 100 μg
ml⁻¹; chloramphenicol (Sigma), 10 μg
ml^-1. 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)
buffer, tris(2-carboxyethyl)phosphine hydrochloride (TCEP),
isopropyl-β-D-1-thiogalactopyranoside (IPTG), dithiothreitol (DTT) were
purchased from Gold BioTechnology; L-(+)-arabinose was purchased from Sigma.

Burckhardt R.M., Buckner B.A, & Escalante-Semerena J.C. (2019). Staphylococcus aureus modulates the activity of acetyl-Coenzyme A synthetase (Acs) by sirtuin-dependent reversible lysine acetylation. Molecular microbiology, 112(2), 588-604.

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Purification of Recombinant GPIbα LBD

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Recombinant human GPIbα ligand-binding domain (LBD) proteins were expressed and purified as described previously. 5, 6 DNA primers were supplied by Integrated DNA Technologies (Coralville, IA); cell culture dishes and flasks were from Corning (Corning, NY); ristocetin was purchased from MP Biomedicals (Santa Ana, CA); bovine serum albumin and guanidine hydrochloride were from Millipore-Sigma (Burlington, MA); and Tris (2carboxyethyl) phosphine hydrochloride was from GoldBio (St. Louis, MO).

Legan E.R., Liu Y., Arce N.A., Parker E.T., Lollar P., Zhang X.F, & Li R. (2023). Type 2B von Willebrand disease mutations differentially perturb autoinhibition of the A1 domain. Blood, 141(10).

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