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Anti nkg2a pe

Manufactured by Miltenyi Biotec
Sourced in United States, Germany

Anti-NKG2A-PE is a monoclonal antibody conjugated with the fluorescent dye R-Phycoerythrin (PE). It is used to detect the expression of the NKG2A receptor on the surface of natural killer (NK) cells and a subset of T cells.

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2 protocols using anti nkg2a pe

1

Comprehensive Analysis of NK Cell Reconstitution

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Peripheral blood mononuclear cells (PBMCs) were separated from fresh blood samples by Ficoll-Hypaque gradients (Sigma-Aldrich, St. Louis, MO, USA) and processed within 4 h. The reconstitution of NK cells after HSCT was determined by eight-color multiparameter flow cytometry using the following mAbs: anti-CD45-FITC (clone; HI30, BD bioscience, San Diego, CA, USA), anti-CD3-V450 (clone; UCHT1, BD bioscience, San Diego, CA, USA), anti-CD16-V500 (clone 3G8; BD bioscience, San Diego, CA, USA), anti-CD56-PE-Cy7 (clone; B159, BD bioscience, San Diego, CA, USA), anti-NKGD-APC (clone; 1D11, BD bioscience, San Diego, CA, USA), anti-NKG2A-PE (clone; REA110, Miltenyi Biotec, Bergisch Gladbach, Germany), anti-NKG2C-Alexa700 (clone 134591, R&D Systems Inc., Minneapolis, MN, USA), and anti-CD57-V450 (clone; TB01, ebioscience, San Diego, CA, USA). PBMCs were also intracellularly stained with anti-CD3ζ-PE (clone; 6B10.2, BD bioscience, San Diego, CA, USA) and anti-FcεRIγ-FITC (FcRγ) (Millipore, Merck Millipore, Burlington, MA, USA) for the analysis of FcεRIγ-deficient NK cells (g-NK cells). NK cell gating and analyses of the NK subpopulation are shown in Figure A1. Flow cytometry data were collected on an FACS Fortessa instrument (BD bioscience, San Jose, CA, USA), using FlowJo version 10.0.6 software (Tree Star, Ashland, OR, USA).
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2

Multiparametric Flow Cytometry for Lymphocyte Subsets

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Whole blood (100 μL) was stained with monoclonal antibodies. Anti-CD45-FITC (clone; HI30), anti-CD3-V450 (clone; UCHT1), anti-NKGD-APC (clone; 1D11), and anti-CD56-PE-Cy7 were purchased from Becton Dickinson (San Jose, CA, USA). Anti-NKG2A–PE was obtained from Miltenyi Biotec (Bergisch Gladbach, Germany). Data were acquired using a Fortessa flowcytometry (BD Biosciences, San Jose, CA, USA) and analyzed using FlowJo version 10.0.6 software (Tree Star, Ashland, OR, USA) (Figure 2). Lymphocyte count in whole blood was measured with Sysmex XN (Sysmex, Kobe, Japan).
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