Anti mek1 2 antibody

Manufactured by Cell Signaling Technology

The Anti-MEK1/2 antibody is a primary antibody that specifically recognizes the MEK1 and MEK2 proteins. MEK1/2 are serine/threonine protein kinases that play a key role in the MAPK/ERK signaling pathway. This antibody can be used for the detection and analysis of MEK1/2 in various research applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Anti-phospho-MEK1/2(Ser217/221) antibody Anti-MEK1/2 MEK1/2 Anti-MEK1

3 protocols using anti mek1 2 antibody

Synthesis and Evaluation of 3-Acyl Isoquinolin-1(2H)-one Compound 4f

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 3-acyl isoquinolin-1(2H)-one complex 4f was synthesized according to a known procedure developed in our previous work. Cell counting kits-8 (CCK8) was purchased from Beyotime Biotechnology, dimethyl sulfoxide (DMSO) and RIPA lysis buffer were purchased from Sigma (Beverly, MA, USA). Primary antibodies such as anti-caspase3 antibody (cat#14220T), anti-cleaved-caspase9 antibody (cat#52873), anti-cleaved-caspase7 antibody (cat#8438), anti-parp antibody (cat#9542), anti-bcl-2 antibody (cat#15071T), anti-bax antibody (cat#5023), anti-β-tubulin antibody (cat#2128s), anti-ERK1/2 antibody (cat#4695T), anti-p-ERK1/2 antibody (cat#4370T), anti-MEK1/2 antibody (cat#9126s), anti-p-MEK1/2 antibody (cat#9154T), (HRP)-labeled anti-rabbit secondary antibody (Cat#7074) and (HRP)-labeled anti-mouse secondary antibody (Cat#7076) were purchased from Cell Signaling Technology. Anti-GSDME antibody (ab215191), anti-GSDMD antibody (ab210070), anti-p-MLKL antibody (ab187091) and anti-MLKL antibody (ab184718) were purchased from Abcam. Anti-CDK1 antibody (AF1516) was purchased from Beyotime Biotechnology.

Ma L., Bian M., Gao H., Zhou Z, & Yi W. (2022). A novel 3-acyl isoquinolin-1(2H)-one induces G2 phase arrest, apoptosis and GSDME-dependent pyroptosis in breast cancer. PLoS ONE, 17(5), e0268060.

+ Open protocol

+ Expand

Western Blot Analysis of MAPK Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor lysates prepared for RPPA were also used for Western blot. Briefly, 20μg protein from each sample was used for 10% polyacrylamide gel electrophoresis, followed by transferring onto nitrocellulose membrane (Biorad, Cat. # 162–0115), blocking with Odyssey Blocking Buffer (PBS) (LI-COR, Lincoln, NE, Cat. # 927–40000) at room temperature for 1h, incubating with primary antibodies at room temperature for 1h and Infrared (IR)-labeled secondary antibodies (IRdye800CW goat anti-rabbit IgG, Cat. 92532211) at room temperature for 1h. Membranes were imaged and quantified using Odyssey Infrared Imaging System and its application software Version 3.0.30 (LI-COR). Newblot Nitro Striping Buffer (LI-COR, Cat. #928–40030) was used for antibody striping and subsequent probing with a different antibody. All first antibodies used are from Cell Signaling Technology (CST): anti-Phospho-MEK1/2 (Ser217/221) antibody (Cat. #9121, 1:1000); anti-MEK1/2 antibody (Cat. #9122, 1:1000); anti-Phospho-p44/42MAPK (Erk1/2) (Thr202/Try204) antibody (Cat. # 4377, 1:1000); and anti-p44/42 MAPK (Erk1/2) antibody (Cat. #9102, 1:1000). Odyssey Protein Molecular Weight Marker (LI-COR, Cat. 928–40000) was used as molecular weight reference.

Bu W., Liu Z., Jiang W., Nagi C., Huang S., Edwards D.P., Jo E., Mo Q., Creighton C.J., Hilsenbeck S.G., Leavitt A.D., Lewis M.T., Wong S.T, & Li Y. (2018). Mammary precancerous stem and non-stem cells evolve into cancers of distinct subtypes. Cancer research, 79(1), 61-71.

+ Open protocol

+ Expand

Western Blot Analysis of MAPK Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed with SDS lysis buffer (0.1 M Tris‐HCl at pH 8.0, 10% glycerol, 1% SDS) and immediately boiled for 10 minutes to obtain clear lysates. Protein concentrations were measured using the BCA method (Pierce). Lysates containing equal amounts of proteins were separated by SDS‐PAGE and transferred to PVDF membranes (Merck) for Western blot analysis using the appropriate antibodies. Immunoreactive proteins were visualized using the Immobilon Western chemiluminescent HRP substrate (Merck) or Clarity Western ECL substrate (Bio‐Rad); light emission intensity was quantified using an LAS‐3000 lumino‐image analyzer equipped with Image Gauge v2.3 software. The antibodies used in this study were: anti‐BRAF antibody (14814; Cell Signaling Technology), anti‐CRAF antibody (53745; Cell Signaling Technology), anti‐MEK1/2 antibody (8727; Cell Signaling Technology), anti‐phospho‐MEK1/2 (Ser217/221) antibody (9121; Cell Signaling Technology), anti‐p44/42 MAPK (ERK1/2) antibody (4695; Cell Signaling Technology), anti‐phospho‐p44/42 MAPK (ERK1/2) (Thr202/Tyr204) antibody (4377; Cell Signaling Technology), anti‐RAS antibody (8955; Cell Signaling Technology), anti‐CRBN antibody (71810; Cell Signaling Technology), and anti‐β‐actin antibody (A5316; Merck).

Ohoka N., Suzuki M., Uchida T., Tsukumo Y., Yoshida M., Inoue T., Ohki H, & Naito M. (2022). Development of a potent small‐molecule degrader against oncogenic BRAFV600E protein that evades paradoxical MAPK activation. Cancer Science, 113(8), 2828-2838.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!