Mir 148b 3p mimics

GenePharma (Shanghai, China) designed and constructed small interfering RNAs (siRNAs) that targeted HOXA11‐AS (si‐HOXA11‐AS), and negative control siRNAs (si‐NC). RiboBio (Guangzhou, China) designed and constructed miR‐148b‐3p mimics, microRNA mimic control (miR‐NC), miR‐148b‐3p inhibitor, and anti‐microRNA inhibitor control (anti‐miR‐NC). Overexpression vectors (HOXA11‐AS, PKM2) and empty vectors (Vector) were synthesized by SyngenTech. We used Lipo3000 (Thermo Fisher) for transfection according to the manufacturer's protocol. The siRNA sequences are listed in Table S1.

miR-148b-3p mimics, miR-148b-3p inhibitor, mimic control and inhibitor control were obtained RiboBio Co. (Guangzhou, Guangdong, China). KIT-coding sequences without the 3′-UTR were cloned into the GV141 vector by GeneChem (Shanghai, China) (called pGV141-KIT). The psiCHECK-2 vector (C8021) was purchased from Promega (Madison, WI, USA). The psiCHECK-2-KIT-wt, psiCHECK-2-KIT-mut-1378, and psiCHECK-2-KIT-mut-1639 were constructed by Bios Biological (Wuhan, Hubei, China). miR-148b-3p agomir and miR agomir NC were generated by GenePharma Co., Ltd. (Shanghai, China). Lipofectamine 3000 (L3000015, Invitrogen, Grand Island, NY, USA) was used to transfect cells with the desired genes or plasmids.