Cell viability of uninfected and HIV‐1‐infected MDMs was monitored over time (longitudinal) and at the end of culture (endpoint) using CytoTox‐One Homogeneous Membrane Integrity Assay or CytoTox‐Glo kits (Promega) per the manufacturer's instructions.
Cytotox glo kit
Manufactured by Promega
Sourced in United States
The CytoTox Glo™ kit is a luminescent-based assay designed to measure the release of cytosolic enzyme lactate dehydrogenase (LDH) from damaged cells. The kit provides a quick, quantitative measure of cell viability or cytotoxicity in a variety of cell types.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using cytotox glo kit
1
Assessing HIV-1 Infected Macrophage Viability
2
Monitoring HIV-1-Infected Macrophage Viability
3
Cytotoxicity Evaluation of AgNPs using MTT and CytoTox Glo Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cytotoxicity was evaluated using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and the CytoTox Glo™ kit from Promega. HaCaT cells were seeded in 96-well plates at a concentration of 2 × 104 cells per well. The cells were treated with varying concentrations of the AgNPs while including tamoxifen and cisplatin (as positive controls) for 24 h. After treatment, 20 μl MTT (0.5 mg/ml) was added to each well and allowed to further incubate in the CO2 incubator for 4 h. Hereafter, the MTT containing medium in each well was discarded and replaced with 100 μl DMSO to dissolve the blue formazan crystals where after absorbance was read at 570 nm in a multi-well ELISA microplate reader. The Cyto Tox Glo™ luminescent assay measures proteases that leaked from compromised cell membranes of dead cells into the culture media. The Manufacturer's protocol instruction was strictly adhered to for the determination of the cell viability (Promega).
4
Evaluating HSP70 Mutant's Sensitivity to VER155008
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
293T cells expressing wild-type Strep-HSP70, S385A/S400A or S385D/S400D protein were seeded in 96-well white plates and cultured overnight. The cells were treated with different concentrations of VER155008 (2.5, 5, 10, and 20 μM) for 6 h, and the dead cells were evaluated with the CytoTox-Glo kit (Promega). After adding a mixture of AAF-Glo substrate and buffer, the cells were incubated at 37 °C for 10 min, and the luminescence signal from the dead cells was measured by a luciferase reader (GloMax, Promega). The experiments were independently repeated three times.
5
Cytotoxicity of Plant Extract on Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HeLa (cervix adenocarcinoma), MCF-7 (breast adenocarcinoma) and H1299 (non-small cell lung adenocarcinoma) human cancer cell lines were employed in this research. The cells were cultured in RPMI 1640 medium in a CO 2 incubator. 24 hours incubation after seeding into 96-well plates (2×10 3 cells/well), the medium was removed from the well leaving the adherent cells and cells were applied with extract for 72 hours in the range of 25-1000 µg/mL. After time was up, cell viability was determined by using CytotoxGlo kit (Promega, USA), in accordance with the manufacturer's instructions. The percentage of cell viability was calculated relative to control cells. A plot of cell viability (%) against concentration was created, and the concentration of the plant extract that decreased cell viability by 50% (IC 50 ) was calculated.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!