The morphology of 15 roots (five plants, three primary roots per plant) was analyzed by hand cross-sectioning into ≈15 mm segments at the middle of the root using a vibratome (Leica VT 1200 S, Germany) and observed under confocal laser scanning microscopy. For elemental analysis plants were separated into roots and shoots, rinsed and cleaned three times in 0.5 mM CaCl2 to remove traces of growth medium from surfaces. Tissues were dried at 60 °C for five days, weighed, homogenized and analyzed for elemental content. Nitrogen and carbon were determined by combustion (TruSpec CHN analyzer, LECO, MI). Results represent averages of 10 plants. All growth experiments were repeated three times.
Truspec chn analyzer
Manufactured by Leco
The TruSpec CHN analyzer is a laboratory instrument designed to determine the carbon, hydrogen, and nitrogen content of solid and liquid samples. It utilizes a combustion-based method to analyze the elemental composition of the sample. The TruSpec CHN analyzer provides accurate and reliable results for a wide range of materials, making it a versatile tool for various scientific and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using truspec chn analyzer
1
Root Morphology and Elemental Analysis
2
Biomass Elemental Composition Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The carbon (C), hydrogen (H), and nitrogen (N) content in biomass, solid residues, and bio-oil were assessed by a LECO TruSpec CHN analyzer, whilst a LECO CNS2000 analyzer determined sulfur (S) content.
3
Soil Decomposition Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The total carbon and total nitrogen content of the initial and decomposed samples was determined by combustion at 650 • C using an elemental LECO TruSpec CHN-analyzer. For the incubated samples, one bag per plot for each material was randomly selected and the content was ground as described above for the fibre analysis. The total C and N analysis was performed in triplicates (ca. 100 mg of ground samples per replicate). A multipoint standard calibration of the instrument was performed using set-up standards recommended by the manufacturer (LECO, USA) prior to the analysis of the granulated biogas residue and rooibos tea samples. The remaining absolute amounts of total carbon and nitrogen after decomposition were calculated on a DM basis and expressed as a percentage of the initial total carbon and nitrogen content of the unexposed material according to Eq. ( 5):
where %R E is the relative residual element (total carbon or total nitrogen) as a percentage of the element initially contained in the nonincubated material, D E is the remaining mass of the element after decomposition in the soil, and I E is the initial mass of the element contained in the unexposed material (Table 3).
where %R E is the relative residual element (total carbon or total nitrogen) as a percentage of the element initially contained in the nonincubated material, D E is the remaining mass of the element after decomposition in the soil, and I E is the initial mass of the element contained in the unexposed material (Table 3).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!