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Mouse zonulin elisa kit

Manufactured by MyBioSource
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The Mouse Zonulin ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of mouse zonulin levels in biological samples.

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Lab products found in correlation

Calprotectin, by R&D Systems (1 mentions) Lipopolysaccharides (lps), by MyBioSource (1 mentions)

Close spelling variants of this product

Zonulin ELISA Kit Zonulin Mouse ELISA kit ELISAs

2 protocols using mouse zonulin elisa kit

1

Biochemical Markers in Mouse Serum

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The whole blood was collected from experimental mice by heart puncture and the serum was prepared by centrifugation at 2000× g for 15 minutes. The specimens were then stored at −80 °C until analysis. The GSH/GSSG ratio was determined using a GSH/GSSG Ratio Detection Assay Kit (Fluorometric‐Green) (#ab138881) from Abcam, while DAO activity was measured by DAO ELISA Kit (#MBS160374) and the zonulin levels were determined by Mouse Zonulin ELISA Kit (#NC1314884) from MyBioSource according to the manufacturers’ instructions.21, 41
Zeng J., Liang Y., Sun R., Huang S., Wang Z., Xiao L., Lu J., Yu H, & Yao P. (2022). Hematopoietic stem cell transplantation ameliorates maternal diabetes–mediated gastrointestinal symptoms and autism‐like behavior in mouse offspring. Annals of the New York Academy of Sciences, 1512(1), 98-113.
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2

Quantification of Intestinal Immunoglobulins

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To quantify luminal Igs, small intestines and colon were cut open longitudinally and mucus and feces were scrapped out and suspended into 1ml of PBS. Luminal content were spun at 8000g for 5 minutes and the supernatant collected and stored at −80°C. LPS (MyBiosource), sIgA (Cloud-Clone Corp.), IgA (Bethyl), IgM (Bethyl), IgG (Bethyl) and Calprotectin (S100A8/S100A9 Heterodimer Duoset; R&D systems) concentrations were measured by ELISAs in the serum or luminal content according to the manufacturer instructions. For luminal Igs measurement, concentration was normalized to the mucus weight. Murine serum Zonulin concentrations were measured by using a mouse Zonulin ELISA kit (MyBioSource).
Rivas M.N., Wakita D., Franklin M.K., Carvalho T.T., Abolhesn A., Gomez A.C., Chen S., Lehman T.J., Sato K., Shibuya A., Fasano A., Kiyono H., Abe M., Tatsumoto N., Yamashita M., Crother T.R., Shimada K, & Arditi M. (2019). Intestinal permeability and IgA provoke immune vasculitis linked to cardiovascular inflammation. Immunity, 51(3), 508-521.e6.
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