Bond rx automatic stainer

Manufactured by Leica

The Bond RX automatic stainer is a piece of lab equipment designed for automated slide staining. It performs various staining protocols to prepare samples for microscopic analysis.

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Lab products found in correlation

Nanozoomer digital pathology system nanozoomer 2.0 ht slide scanner, by Hamamatsu Photonics (1 mentions) Bond polymer refine detection, by Leica (1 mentions) Scanscope xt microscope, by Leica (1 mentions) Aperio imagescope software, by Leica (1 mentions)

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Bond RX (391 citations) BOND automatic stainer (3 citations) Automatic stainer (2 citations) Leica stainer (2 citations)

3 protocols using bond rx automatic stainer

Histological Analysis of RCCEP Lesions

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The RCCEP lesion tissues were fixed with 10% neutral formaldehyde, dehydrated with an alcohol gradient, infiltrated with paraffin wax, and embedded in paraffin to form paraffin-embedded blocks. Sections (4 μm) were cut for hematoxylin and eosin (H&E) staining, immunohistochemical (IHC), and immunofluorescence staining (Multiplex IF). The sections were baked at 60 °C overnight, and then IHC and Multiplex IF staining were carried out using a Bond RX automatic stainer (Leica Biosystems, Buffalo Grove, IL). The stained sections were scanned using NanoZoomer Digital Pathology System (Nanozoomer 2.0-HT slide scanner (Hamamatsu, Hamamatsu City, Japan). The NDP view imaging software (NDP scan software) was used to analyze the scan results and catch representative images.

Wang F., Qin S., Sun X., Ren Z., Meng Z., Chen Z., Chai X., Xiong J., Bai Y., Yang L., Zhu H., Fang W., Lin X., Chen X., Li E., Wang L., Yan P, & Zou J. (2020). Reactive cutaneous capillary endothelial proliferation in advanced hepatocellular carcinoma patients treated with camrelizumab: data derived from a multicenter phase 2 trial. Journal of Hematology & Oncology, 13, 47.

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Immunohistochemical Analysis of BTNL8 in Duodenal Tissue

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Duodenal biopsies were preserved in formalin and embedded in paraffin. Sections were cut to a thickness of 5 μm and stained with Bond RX Automatic Stainer (Leica Biosystems). Slides were then dewaxed three times with xylene, ethanol, and water, treated for 20 min with Epitope Retrieval Solution II (Leica Biosystems), incubated for 5 min with 0.5% casein to prevent non-specific binding, and stained for 1 hr with a 1:200 dilution of αBTNL8 (2187B, R&D Systems). Antigen-antibody binding was revealed using Bond Polymer Refine Detection (Leica Biosystems). Tissue sections were then blocked with peroxidase, stained with DAB, and counter-stained with hematoxylin. Images were acquired using a ScanScope XT microscope (Leica Biosystems).

Mayassi T., Ladell K., Gudjonson H., McLaren J.E., Shaw D.G., Tran M.T., Rokicka J.J., Lawrence I., Grenier J.C., van Unen V., Ciszewski C., Dimaano M., Sayegh H.E., Kumar V., Wijmenga C., Green P.H., Gokhale R., Jericho H., Semrad C.E., Guandalini S., Dinner A.R., Kupfer S.S., Reid H.H., Barreiro L.B., Rossjohn J., Price D.A, & Jabri B. (2019). Chronic Inflammation Permanently Reshapes Tissue-Resident Immunity in Celiac Disease. Cell, 176(5), 967-981.e19.

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CTR1 Immunohistochemical Profiling of Tumor Samples

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The TMAs were sectioned at 4µm and immunohistochemistry (IHC) was performed using a BOND-RX automatic stainer (Leica Biosystem). Slide deparaffinization was performed with BOND Dewax solution followed by alcohol dehydration. Epitope retrieval was performed using HIER2 Buffer (BOND, AR9540) and slides were incubated for 60 min at room temperature using a rabbit monoclonal anti-CTR1 primary antibody (Abcam, ab133385) at 1:100 dilution in Primary Antibody Diluent (BOND, AR9352). Staining was visualized using a Polymer Refine Detection kit (BOND, DS9800) and nuclei were counterstained with haematoxylin. Tumors cells showing CTR1 membrane staining (or a combination of membrane and cytoplasmic staining) of any intensity were considered CTR1-positive. The slide was scanned using an Aperio CS2 virtual microscope (Leica Biosystem) and each core was analyzed using the Positive Pixel Count function of the Aperio Image Scope software (version 9.1, Leica Biosystem). CTR1 staining was scored according to intensity-weighted positive-pixel counting (PPC) methodology as basal CTR1 levels, similar to control liver (<10 PPC*1000/µm2), elevated (+), similar to control kidney (10-25 PPC*1000/µm2), intermediate elevated (++) staining (>25 PPC*1000/µm2) or strongly elevated (+++) staining (>40 PPC*1000/µm2).

Parmar A., Pascali G., Voli F., Lerra L., Yee E., Ahmed-Cox A., Kimpton K., Cirillo G., Arthur A., Zahra D., Rahardjo G., Liu G.J., Lengkeek N., Saletta F., Charil A., Kavallaris M, & Vittorio O. (2018). In vivo [64Cu]CuCl2 PET imaging reveals activity of Dextran-Catechin on tumor copper homeostasis. Theranostics, 8(20), 5645-5659.

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