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N acetylcystein

Manufactured by MedChemExpress
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N-acetylcystein is a chemical compound used in laboratory settings. It serves as a reducing agent and is commonly employed in various chemical reactions and analytical procedures.

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2 protocols using n acetylcystein

1

Cytotoxicity Assay with CM-H2DCFDA and MTT

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CM-H2DCFDA was purchased from Molecular probes (Invitrogen, Eugene, OR, USA); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and propidium iodide (PI)—from Sigma (Taufkirchen, Germany). cOmplete™ EASYpacks protease inhibitors cocktail and PhosSTOP™ EASYpacks phosphotase inhibitors cocktail—from Roche (Mannheim, Germany). Anisomycin—from NeoCorp (Weilheim, Germany). N-acetylcystein—from MedChemExpress (Monmouth Junction, NJ, USA). Docetaxel, cabazitaxel, cisplatin and carboplatin—from a Pharmacy of the University Hospital Hamburg-Eppendorf (Hamburg, Germany). RNase—from Carl Roth (Karlsruhe, Germany). Primary and secondary antibodies are listed in Table 2.
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2

Annexin-V-FITC Apoptosis Assay Protocol

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Annexin-V-FITC was purchased from BD Bioscience (San Jose, CA, USA). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and propidium iodide (PI) from Sigma (Taufkirchen, Germany). cOmplete™ EASYpacks protease inhibitors cocktail and PhosSTOP™ EASYpacks – from Roche (Mannheim, Germany). Anisomycin was from NeoCorp (Weilheim, Germany). z-VAD(OMe)-fmk was from Enzo Life Sciences (Farmingdale, NY, USA). H2O2 was from Carl Roth (Karlsruhe, Germany). CCCP (2-[2-(3-chlorophenyl)hydrazinylyidene]propanedinitrile) was from Sigma (Taufkirchen, Germany). JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide) was from AdiloGen Life Science (Epalinges, Switzerland). N-acetylcystein was from MedChemExpress (Monmouth Junction, NJ, USA). Primary and secondary antibodies used are listed in Table 1.
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