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Siporter sirna transfection kit

Manufactured by Thermo Fisher Scientific
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The SiPORTER siRNA transfection kit is a laboratory tool designed for the efficient delivery of small interfering RNA (siRNA) into cells. It facilitates the introduction of siRNA molecules into the target cells, enabling the study of gene function through RNA interference (RNAi) techniques.

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SiRNA Transfection Kit (3 citations) SiRNAs (258 citations)

2 protocols using siporter sirna transfection kit

1

AMPK-α1 Silencing in HRMECs

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HRMECs were transfected by an siPORTER siRNA transfection kit (Cat#AM4503, Ambion) per manufacturer’s protocol with siRNA duplex against AMPK-α1 and universal negative control (Santa Cruz). Briefly, HRMECs were plated on six-well plates. After HRMECs reached 80% confluence, siRNA was applied to each well (final concentration 50 nM). Protein suppression was evaluated by Western blot 72 h later.
Yan Z., Wang C., Meng Z., Gan L., Guo R., Liu J., Bond Lau W., Xie D., Zhao J., Lopez B.L., Christopher T.A., Naik U.P., Ma X, & Wang Y. (2022). C1q/TNF-Related Protein 3 Prevents Diabetic Retinopathy via AMPK-Dependent Stabilization of Blood–Retinal Barrier Tight Junctions. Cells, 11(5), 779.
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2

siRNA-Mediated AMPK-α1 Silencing in HRMECs

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siRNA duplex oligonucleotides were designed for AMP kinase-α1 (AMPK-α1) target sequences, silencing respective gene expression. HRMECs were transfected by siPORTER siRNA transfection kit (Cat, AM4503, Ambion) per manufacturer’s protocol with siRNA duplex against AMPK-α1 (5’-UGCCUACCAUCUCAUUAdTdT-3’) and universal control (Santa Cruz). Briefly, HRMECs were plated on six-well plates before transfection. After reaching 80% confluence, siRNA was applied to each well (final concentration 50 nM).
Yan Z., Zhao J., Gan L., Zhang Y., Guo R., Cao X., Lau W.B., Ma X, & Wang Y. (2017). CTRP3 is a novel biomarker for diabetic retinopathy and inhibits HGHL-induced VCAM-1 expression in an AMPK-dependent manner. PLoS ONE, 12(6), e0178253.
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