Chinese Hamster Ovary (CHO-K1) cells (ATCC CCL-61 or CRL-9618) were grown in Ham’s F-12 medium (Corning) containing 10% heat inactivated fetal bovine serum complex (FBS) (Corning) and 1% penicillin-streptomycin (100 U penicillin/mL, 100 μg streptomycin/mL; Sigma). Human embryonic kidney 293 (HEK293) cells (ATCC CRL-1573) or HEK293-T14 cells (HEK293T cells stably expressing T7 RNA polymerase, ATCC CRL-3216) were grown DMEM with 100 μg/mL zeocin (Invitrogen, for HEK293T cells expressing T7 RNA polymerase only), respectively containing 10% heat inactivated FBS and 1% penicillin-streptomycin. Detroit 562 cells (ATCC CCL138) were grown in MEM medium (Corning) containing 10% heat inactivated fetal bovine serum complex (FBS) (Corning) and 1% penicillin-streptomycin (100 U penicillin/mL, 100 μg streptomycin/mL; Sigma). CHO-K1, HEK283, HEK293T, AGS, and Detroit 562 cells were obtained from ATCC and routinely tested for mycoplasma using LookOut® Mycoplasma PCR Detection Kit from Sigma. The cell lines were authenticated by ATCC and not authenticated in our laboratory.
Fetal bovine serum complex fbs
Manufactured by Corning
Fetal bovine serum complex (FBS) is a laboratory reagent derived from the blood of bovine fetuses. FBS contains a complex mixture of proteins, growth factors, and other components that support the growth and proliferation of cells in cell culture systems. It is commonly used as a supplement in cell culture media to provide essential nutrients and growth factors for cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Lookout mycoplasma pcr detection kit, by Merck Group (2 mentions)
Zeocin, by Thermo Fisher Scientific (2 mentions)
Hek293t, by American Type Culture Collection (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Merck Group (2 mentions)
Ham s f 12 medium, by Corning (2 mentions)
2 protocols using fetal bovine serum complex fbs
1
Cell Culture Protocols for Diverse Cell Lines
2
Cell Culture Protocols for Diverse Cell Lines
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