Rabbit anti krt8

The following antibodies were used: rabbit anti-METTL3 (1:200; Abcam, Cat No: ab195352), rabbit anti-KRT8 (1:200; Abcam, Cat No: ab53280), goat anti- gustducin (1:100; Aviva Systems Biology, Cat No: OAEB00418), rabbit anti-PGP9.5 (1:500, Thermo Fisher, Cat No: 480012), rabbit anti-SNAP25 (1:100; Proteintech, Cat No: 14903-1-AP), mouse anti- PAN-CK(1:100, Thermo Fisher, Cat No: MA1-82041), mouse anti-KRT13 (1:200; Abcam, Cat No: ab16112), rabbit anti-p63 (1:300; Abcam, Cat No: ab53039), mouse anti-LATS1 (1:100; Santa Cruz Biotechnology, Cat No: sc-398560), mouse anti-YAP (1:200; Santa Cruz Biotechnology, Cat No: sc-101199), mouse anti-TAZ (1:100; Santa Cruz, Cat No: sc-293183), rabbit anti-FZD7 (1:1 000 for western blot; Abcam, Cat No: ab64636), rabbit anti-β-catenin (1:250; Proteintech, Cat No: 51067-2-AP), rabbit anti-LEF1 (1:200; Abcam, Cat No: ab137872).

Tissues were fixed in zinc-formalin, paraffin-embedded and sectioned at 5 microns, and were stained with Hematoxylin and Eosin (H&E), or prepared for immunostaining as previously described [61 (link), 72 (link)]. Images were captured with 10, 20 or 40x objectives, using a Nikon Eclipse NI-U with a DS-QI1 or DS-Ri1 camera and NIS Elements software, and adjusted in Adobe Photoshop. Antibodies were as follows: Rabbit and chicken anti-Krt5, mouse anti-Krt8 (Covance), rabbit anti-Krt8 (Abcam), rabbit anti-Ki-67 (Abcam), rabbit anti-AR (AR-441; Abcam), mouse anti-p63 (Biocare medical), rabbit anti-Syp (Thermo Fisher Scientific), rabbit anti-Krt10 (Covance), rabbit anti-Tgfbr2 (Novus), mouse anti-pAkt (Cell Signaling), rabbit anti-pSmad2 (Millipore), rabbit anti-Smad4 (Millipore) and chicken anti-GFP (Abcam). Alexafluor 488, 546 and 647 secondary antibodies were from Invitrogen. DNA was stained with Hoechst 33342. Confocal images were captured using a Zeiss LSM710 Multiphoton Confocal microscope and adjusted in Adobe Photoshop. Two tissue microarrays (TMA) contained four 0.6mm tissue cores from each of 93 cancers from patients who had radical prostatectomy. A third TMA contained four 0.6mm cores from each of 45 cancers from TURP samples. TMAs were examined by IHC, as in [20 (link)], or by IF for Krt5 and Krt8. Following IF and imaging, the same slide was stained with H&E.