Chromatin immunoprecipitation was carried out on T‐ALL cells as described.40 DNA was immunoprecipitated with anti‐HDAC1 (sc‐7872X,) anti‐HDAC2 (sc‐7872X), anti‐HDAC3 (sc‐11417X), anti‐MeCP2 (sc‐137070), nonspecific IgG Abs (Santa Cruz Biotechnology, Dallas, TX, USA) or MBD3 (D1B8F) (Cell Signaling Technology, Danvers, MA, USA) and amplified by qRT‐PCR (Table S3 ) using SYBR Green PCR Master Mix on a StepOnePlus Real‐Time PCR System under standard conditions. Results were quantified by SYBR Green Real‐Time PCR analysis. The fold enrichment of immunoprecipitated samples was normalized on INPUT and expressed relative to the mock‐treated control (IgG). Results were visualized after separating PCR products on 3% agarose gel stained with GelGreen Nucleic Acid Staining.
Anti mecp2
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-MeCP2 is a laboratory reagent designed to detect the presence of the MeCP2 protein in biological samples. MeCP2 is a key regulator of gene expression, and its detection can provide valuable insights into various biological processes. This product is intended for research use only and should not be used for diagnostic or therapeutic purposes.
Automatically generated - may contain errors
Lab products found in correlation
Anti hdac3, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by GE Healthcare (1 mentions)
Anti actin, by Proteintech (1 mentions)
Fusion fx5 system, by Vilber (1 mentions)
Anti dnmt1, by Abcam (1 mentions)
Anti dnmt3a, by Cell Signaling Technology (1 mentions)
Anti dnmt3b, by Santa Cruz Biotechnology (1 mentions)
Anti mbd2, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti mecp2
1
Chromatin Immunoprecipitation of Epigenetic Regulators
2
Immunoblotting of DNA Methylation Regulators
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The cells were lysed in a lysis buffer. Equal amounts of total protein were loaded onto 4–12% SDS–PAGE gels and transferred onto PVDF membranes (GE Healthcare Life Sciences). The membranes were blocked with 5% skim milk dissolved in TBS containing 0.02% Tween 20 and incubated overnight at 4 °C with specific primary antibodies. The membranes were subsequently incubated with horseradish peroxidase-conjugated secondary antibodies. Protein bands were visualized using the Fusion FX5 system (Vilber Lourmat). The following primary antibodies were used: anti-DNMT1 (Abcam), anti-DNMT3A (Cell Signaling Technology), anti-DNMT3B (Santa Cruz), anti-DNMT3L (Santa Cruz), anti-MBD4 (Santa Cruz), anti-MBD2 (Santa Cruz), anti-MeCP2 (Santa Cruz), and anti-Actin (Proteintech).
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