Western images were developed using the Licor Odyssey (Image Studio 5.2.5). For immunocytochemistry/immunohistochemistry, images were taken on a Nikon TE2000 inverted microscope using a Nikon Digital Sight DS-Qil camera and Nikon NIS Elements BR 4.6 software. Images of any type were brightened or contrasted as a whole, never in part. In Westerns, species specific far red secondaries were used to visualize signals for the FLAG tag (green) and r5-HT7 receptor (red) on the Licor Odyssey. For imaging of cells or tissues, FITC was the fluorophore used to track the r5-HT7 while TRITC tracked the FLAG tag. For immunocytochemistry, some cellular images were enlarged from original images to more closely identify cellular staining and demonstrate TRITC signaling that was difficult to observe with the strong FITC (r5-HT7) signal.
Digital sight ds qil
Manufactured by Nikon
The Digital Sight DS-Qil is a laboratory imaging system designed for microscope-based digital imaging applications. It features a high-resolution CMOS sensor, advanced image processing capabilities, and a user-friendly software interface for capturing, analyzing, and managing digital images. The DS-Qil provides reliable and consistent image quality, making it suitable for a variety of scientific and research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using digital sight ds qil
1
Visualizing Cell Surface Receptor Expression
2
Histological Analysis of Thoracic Aorta
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Immediately upon dissection, the 2 mm rings of thoracic aorta were formalin (10%) fixed and paraffin embedded by MSU Investigative Histopathology. Sections (8 micron thick) were cut and tissues stained standardly with Masson trichrome stain. Sections were photographed on a Nikon TE2000 inverted microscope using a Nikon Digital Sight DS-Qil camera and Nikon NIS Elements BR 4.6 software. Settings were used consistently across all images.
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