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Fish dopamine elisa kit

Manufactured by Cusabio
Sourced in China

The Fish Dopamine ELISA Kit is a quantitative in vitro enzyme-linked immunosorbent assay designed for the measurement of dopamine levels in fish samples.

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2 protocols using fish dopamine elisa kit

1

Serum Dopamine ELISA Measurement

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Serum dopamine was measured using a Fish Dopamine ELISA Kit (Cusabio, Wuhan, China), following the manufacturer’s instructions. A Synergy H4 Hybrid Multi-Mode Microplate Reader (BioTek, Winusky, USA) was used to detect the optical density per well at 450 nm within 10 min.
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2

Dopamine Measurement by ELISA

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Dopamine in serum and culture medium was measured by indirect enzyme linked immunosorbent assay (ELISA) as described by Wu et al.50 using the Fish Dopamine ELISA Kit (CUSABIO, China) according to the supplier’s instructions. Negative controls (PBS-only and blank) were included in the assay. After the reaction, the optical density of each well was detected at 450 nm within 10 minutes using Synergy H4 Hybrid Multi-Mode Microplate Reader (BioTek, USA). Dopamine concentration of each sample was calculated according to a standard curve7 (link).
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