Anti setdb1 eset

Manufactured by Cell Applications

Anti-SETDB1/ESET is a laboratory-grade antibody that recognizes the SETDB1/ESET protein. SETDB1/ESET is a histone methyltransferase that catalyzes the tri-methylation of histone H3 at lysine 9 (H3K9me3), which is involved in gene silencing and heterochromatin formation. The Anti-SETDB1/ESET antibody can be used for various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of the SETDB1/ESET protein.

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Lab products found in correlation

Anti α tubulin clone b512, by Merck Group (2 mentions) Anti flag m2 antibody, by Merck Group (2 mentions) Anti g9a, by Cell Signaling Technology (1 mentions) Anti v5 antibody, by Thermo Fisher Scientific (1 mentions) Anti h3, by Merck Group (1 mentions) Ab84497, by Abcam (1 mentions) Anti v5 r960 25, by Thermo Fisher Scientific (1 mentions) F7425, by Merck Group (1 mentions)

2 protocols using anti setdb1 eset

Antibody panel for chromatin research

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The following antibodies are used for this study: anti-α-Tubulin (clone B-5-1-2, Sigma-Aldrich); anti-FLAG M2 antibody (F3165, Sigma-Aldrich); anti-V5 antibody (R960-25, Thermo Fisher which is same as #46-0705, Life technology); anti-G9a (#8620,(11)); anti-GLP (#0422,(11)); anti-SUV39H1 (clone D11B6, Cell signalling); anti-MCAF1/ATF7IP [48 (link)] and anti-ATF7IP (ab84497, Abcam); anti-MPP8 (16796-1-AP, Proteintech); anti-H3 (07-690, Millipore); anti-Pan-Kme2 (c4930/c5123 mix.(19)); anti-Pan-Kme3 (76118, Abcam); anti-SETDB1/ESET (cp10377, Cell Applications).

Tsusaka T., Kikuchi M., Shimazu T., Suzuki T., Sohtome Y., Akakabe M., Sodeoka M., Dohmae N., Umehara T, & Shinkai Y. (2018). Tri-methylation of ATF7IP by G9a/GLP recruits the chromodomain protein MPP8. Epigenetics & Chromatin, 11, 56.

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Antibody Screening for Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following antibodies used for this study: anti-α-Tubulin (clone B-5-1-2, Sigma-Aldrich); anti-FLAG M2 antibody (F3165, Sigma-Aldrich for western blot and IF assays; F7425, Sigma-Aldrich for IF analysis); anti-MCAF1/ATF7IP (ab84497, Abcam); anti-SETDB1/ESET (CP10377, CELL APPLICATIONS, for western blot and IF analyses); anti-V5 (R960-25, Thermo Fisher which is the same as #46-0705, Life technology); anti-ZMYM2 (A301-711A, Bethyl Laboratories).

Tsusaka T., Fukuda K., Shimura C., Kato M, & Shinkai Y. (2020). The fibronectin type-III (FNIII) domain of ATF7IP contributes to efficient transcriptional silencing mediated by the SETDB1 complex. Epigenetics & Chromatin, 13, 52.

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