J.RT3-T3.5 and Jurkat (Clone E6–1) cell lines used for counter selection and nucleofection, respectively, were purchased from ATCC. The Epstein-Barr virus transformed lymphoblastoid cell line (TM-LCL) used in the rapid expansion protocol (REP) of T cells was made from mononuclear cells as previously described.57 CD19+ and OKT3+ K562 cells used for functional assays were generated by lentivirally transducing parental K562 parental cells (ATCC) with CD19- or OKT3-expressing constructs. Raji parental cells were also purchased from ATCC. All the above cell lines were cultured in RPMI 1640 medium (Gibco) with 10% heat-inactivated FBS (Life Tech and VWR). Human peripheral blood mononuclear cells (PBMCs) were isolated from Leukocyte Reduction System (LRS) cones (Bloodworks Northwest) using Ficoll-Paque density gradient centrifugation (GE). Mixed or CD8+ T cells used in non-isolation experiments were a generous gift from Juno Therapeutics. Rhesus PBMCs were a generous gift from Dr. Agne Taraseviciute (Seattle Children’s Research Institute).
Raji parental cells
Manufactured by American Type Culture Collection
Raji parental cells are a stable cell line derived from a Burkitt's lymphoma. They are used as a model system for various biological studies.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Ficoll paque density gradient centrifugation, by GE Healthcare (2 mentions)
Jurkat clone e6 1, by American Type Culture Collection (2 mentions)
J rt3 t3, by American Type Culture Collection (2 mentions)
Lcl, by American Type Culture Collection (2 mentions)
Parental k562 parental cells, by American Type Culture Collection (2 mentions)
2 protocols using raji parental cells
1
Characterization of Immune Cell Lines
2
Characterization of Immune Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
J.RT3-T3.5 and Jurkat (Clone E6–1) cell lines used for counter selection and nucleofection, respectively, were purchased from ATCC. The Epstein-Barr virus transformed lymphoblastoid cell line (TM-LCL) used in the rapid expansion protocol (REP) of T cells was made from mononuclear cells as previously described.57 CD19+ and OKT3+ K562 cells used for functional assays were generated by lentivirally transducing parental K562 parental cells (ATCC) with CD19- or OKT3-expressing constructs. Raji parental cells were also purchased from ATCC. All the above cell lines were cultured in RPMI 1640 medium (Gibco) with 10% heat-inactivated FBS (Life Tech and VWR). Human peripheral blood mononuclear cells (PBMCs) were isolated from Leukocyte Reduction System (LRS) cones (Bloodworks Northwest) using Ficoll-Paque density gradient centrifugation (GE). Mixed or CD8+ T cells used in non-isolation experiments were a generous gift from Juno Therapeutics. Rhesus PBMCs were a generous gift from Dr. Agne Taraseviciute (Seattle Children’s Research Institute).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!