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Sheep anti rabbit secondary antibodies

Manufactured by GE Healthcare
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Sourced in United States

Sheep anti-rabbit secondary antibodies are laboratory reagents used in immunological assays and techniques. They are designed to detect and bind to primary antibodies raised in rabbits, enabling the identification and visualization of target analytes or molecules of interest.

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Lab products found in correlation

Serine p 2481 mtor, by Cell Signaling Technology (3 mentions) Serine 235 236 phospho s6 ribosomal protein, by Cell Signaling Technology (3 mentions) Total mtor, by Cell Signaling Technology (3 mentions) Total akt, by Cell Signaling Technology (2 mentions) P akt serine 473, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

HRP-conjugated donkey anti-rabbit and sheep anti-mouse secondary antibodies (3 citations) Sheep anti-mouse or donkey anti-rabbit secondary antibodies (3 citations) Donkey anti-rabbit and sheep anti-mouse horseradish peroxidase–conjugated secondary antibodies (2 citations)

3 protocols using sheep anti rabbit secondary antibodies

1

Western Blot Antibody Inventory

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Antibodies against the following proteins were purchased from Cell Signaling: total Akt (cat #4691); total mTOR (cat #2983); serine P-2481 mTOR (cat #2976); S6 (cat #2217); serine 235/236 phospho-S6 ribosomal protein (cat #2211). Sheep anti-rabbit secondary antibodies were obtained from G. E. Health Care Bioscience Corp. (Piscataway, NJ, USA).
Soliman G.A., Abzalimov R.R, & He Y. (2022). mTORC1 and mTORC2 Complexes Regulate the Untargeted Metabolomics and Amino Acid Metabolites Profile through Mitochondrial Bioenergetic Functions in Pancreatic Beta Cells. Nutrients, 14(15), 3022.
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2

Antibody Validation for Signaling Pathways

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The following antibodies were purchased from Cell Signaling: Total mTOR (Cat # 2983); Serine P-2481 mTOR (cat # 2976); S6 (Cat # 2217); Serine 235/236 Phospho-S6 ribosomal protein (Cat # 2211); Akt (Cat # 4691); Serine P-473 Akt (Cat # 4060). Actin antibody was obtained from Sigma (Cat # A2103). Sheep anti-rabbit secondary antibodies were obtained from (GE Health Care Bioscience, Corp, Piscataway, NJ).
Soliman G.A., Steenson S.M, & Etekpo A.H. (2016). Effects of Metformin and a Mammalian Target of Rapamycin (mTOR) ATP-Competitive Inhibitor on Targeted Metabolomics in Pancreatic Cancer Cell Line. Metabolomics : open access, 6(3), 183.
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3

Antibody Validation for mTOR and AKT Pathway

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Antibodies against the following proteins were purchased from Cell Signaling: total mTOR (catalog no. 2983), serine P-2481 mTOR (catalog no. 2976), S6 (catalog no. 2217), serine 235/236 phospho-S6 ribosomal protein (catalog no. 2211), total Akt (catalog no. 4691), serine P-473 Akt (catalog no. 4060), as we previously reported (25 ). The actin antibody was obtained from Sigma (catalog no. A2103). Sheep anti-rabbit secondary antibodies were obtained from GE Health Care Bioscience.
Soliman G.A., Shukla S.K., Etekpo A., Gunda V., Steenson S.M., Gautam N., Alnouti Y, & Singh P.K. (2020). The Synergistic Effect of an ATP-Competitive Inhibitor of mTOR and Metformin on Pancreatic Tumor Growth. Current Developments in Nutrition, 4(9), nzaa131.
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