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On targetplus atm sirna

Manufactured by Horizon Discovery
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The On-TARGETplus ATM SiRNA is a laboratory product designed for gene silencing experiments. It is a small interfering RNA (siRNA) molecule that targets the ATM gene, which is involved in DNA damage response pathways. The product is intended for use in cell-based assays to study the effects of ATM gene knockdown.

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Lab products found in correlation

Si genome human pex2, by Horizon Discovery (2 mentions) Pex10 and pex12 sirna, by Horizon Discovery (2 mentions) Si smartpool human pex5, by Horizon Discovery (2 mentions) Si smartpool human sqstm1, by Horizon Discovery (2 mentions) Sirna buffer, by Horizon Discovery (2 mentions) Dharmafect 1, by Horizon Discovery (2 mentions) Dharmafect 4, by Horizon Discovery (2 mentions)

Spelling variants of this product

ON-TARGETplus (235 citations) ON-TARGETplus siRNAs (227 citations) SiRNAs (161 citations)

2 protocols using on targetplus atm sirna

1

Peroxisome Biogenesis Knockdown in HEK 293

Check if the same lab product or an alternative is used in the 5 most similar protocols
On-TARGETplus ATM SiRNA (L-003201-00-0005); Si GENOME human PEX2, PEX10 and PEX12 SiRNA (M-006548-02, M-006545-00, and M-019337-02); si SMARTpool human PEX5 (M-015788-00); si SMARTpool human SQSTM1 (M-010230-00); were purchased from Dharmacon. siRNAs were resuspended in 1X siRNA buffer (GE Dharmacon) to obtain a 20 μM stock, aliquots were then frozen to prevent repeated freeze thawing. HEK 293 cells were transfected with indicated Si RNA at 10 nM final concentrations with DharmaFECT 1 or DharmaFECT 4 (GE Dharmacon) according to manufacturer's instruction, for 72 h before collection of lysates.
Zhang J., Tripathi D.N., Jing J., Alexander A., Kim J., Powell R.T., Dere R., Tait-Mulder J., Lee J.H., Paull T.T., Pandita R.K., Charaka V.K., Pandita T.K., Kastan M.B, & Walker C.L. (2015). ATM Functions at the Peroxisome to Induce Pexophagy in Response to ROS. Nature cell biology, 17(10), 1259-1269.
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2

Peroxisome Biogenesis Knockdown in HEK 293

Check if the same lab product or an alternative is used in the 5 most similar protocols
On-TARGETplus ATM SiRNA (L-003201-00-0005); Si GENOME human PEX2, PEX10 and PEX12 SiRNA (M-006548-02, M-006545-00, and M-019337-02); si SMARTpool human PEX5 (M-015788-00); si SMARTpool human SQSTM1 (M-010230-00); were purchased from Dharmacon. siRNAs were resuspended in 1X siRNA buffer (GE Dharmacon) to obtain a 20 μM stock, aliquots were then frozen to prevent repeated freeze thawing. HEK 293 cells were transfected with indicated Si RNA at 10 nM final concentrations with DharmaFECT 1 or DharmaFECT 4 (GE Dharmacon) according to manufacturer's instruction, for 72 h before collection of lysates.
Zhang J., Tripathi D.N., Jing J., Alexander A., Kim J., Powell R.T., Dere R., Tait-Mulder J., Lee J.H., Paull T.T., Pandita R.K., Charaka V.K., Pandita T.K., Kastan M.B, & Walker C.L. (2015). ATM Functions at the Peroxisome to Induce Pexophagy in Response to ROS. Nature cell biology, 17(10), 1259-1269.
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