Gc ms model qp 2010

For determination of the chemical composition of basil leaves, leaves of peppermint, and clove buds EOs, gas chromatography–mass spectrometry (GC-MS) analysis was conducted using GC-2010 Shimadzu capillary gas chromatography directly coupled to the mass spectrometer system (GC-MS–model QP 2010; (Shimadzu, Kyoto, Japan)) DB-c18 column under the following conditions: The injector temperature was 250 C. Oven temperature program: 30 °C for 2.0 min, then ramp to 250 °C at a rate of 2.0 degrees Celsius per minute for 5.0 min. The MS source temperature was 200 °C, electron energy was 70 eV, the carrier gas was helium at a flow rate of 1.4 mL/min, and 1 µL of each diluted sample in n-hexane (1:1, v/v) was injected. EI spectra were scanned from 43.00 to 600 m/z to identify peaks through NIST mass data-search libraries and the highest REV and similarity indicators’ hits. The sample components were identified by comparing their relative indices and mass spectra with the computer controlling the GC-MS system [37 (link),38 (link)].
Table 3 shows the VOCs in star anise essential oil. The main compound in the essential oil is (E)-Anethole* accounted for 89.24% of oil, followed by lower contents. Volatile organic compounds exhibited powerful potential against parasites [39 (link)].

For determination of the chemical composition of basil leaves, leaves of peppermint and clove bud EO, gas chromatography-mass spectrometry (GC-MS) analysis was conducted using GC-2010 Shimadzu capillary gas chromatography directly coupled to the mass spectrometer system (GC-MS–model QP 2010; (Shimadzu, Kyoto, Japan) DB-c18 column under the following conditions: The injector temperature is 250 °C. Oven temperature program: 30 °C for 2.0 min, then ramp to 250 °C at a rate of 2.0 °C per minute for 5.0 min. The MS source temperature was 200 °C, electron energy was 70 eV, the carrier gas was helium at a flow rate of 1.4 mL/min, and 1 µL of each diluted sample in n-hexane (1:1, v/v) was injected. EI spectra were scanned from 43.00 to 600 m/z to identify peaks through NIST mass data search libraries and the highest REV and similarity indicators hits. The sample components were identified by comparing their relative indices and mass spectra with the computer controlling the GC-MS system [38 (link)].

The supernatant of the selected bacterium was analyzed using gas chromatography; 2010 Shimadzu capillary attached to the mass spectrometer unit (GC-MS–model QP 2010 (Shimadzu, Kyoto, Japan)); DB–5 ms nonpolar fused silica capillary column (30 m × 0.25 mm, 0.25 μm film thickness). The oven temperature system was 70 °C, 3 °C/min gradient to 200 °C for 35 min. Injection temperature at 200 °C with helium as the carrier gas at 1.0 mL/min, and linear velocity at 45.1 cm/s. The effluent of the GC column was presented into the supply of MS and spectra were acquired in the EI mode with ionization energy of 70 eV in the electronic ionization mode. The temperature of the ion source was 200 °C with 3 min of solvent cut time. The scanning of the mass analyzer was set between 40 and 1000 m/z at 240 °C. The sample constituents were identified by comparing the relative indices and mass spectra using libraries of WILEY and the National Institute of Standards and Technology.