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3 protocols using anti e cadherin rat antibody

1

Immunofluorescence Staining for E-cadherin, Myosin-VIIa, and Histone H3

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The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (Thermo Fisher Scientific, 13-1900, 1 : 100 dilution), anti-Myosin-VIIa rabbit polyclonal antibody (Proteus BioSciences Inc., #25-6790, 1 : 200 dilution), anti-Histone H3 (phospho S28) rat polyclonal antibody (Abcam, #ab10543, 1 : 200 dilution), Alexa Fluor 546-conjugated goat anti-rat IgG (H + L) antibody (Thermo Fisher Scientific, #A11081, 1 : 1000 dilution) and Alexa Fluor 647-conjugated goat anti-rabbit IgG (H + L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1 : 1000 dilution). We used MMC (Nacalai Tesque, #20898-2).
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2

Immunofluorescence Labeling of E-cadherin and SOX9

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The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (#13-1900, 1:50 dilution, Thermo Fischer Scientific), anti-SOX9 rabbit antibody (#AB5535-25UG, 1:200 dilution, Merck Millipore), Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (#A11081, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rat IgG (H+L) antibody (#A21247, 1:1000 dilution), Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (#A32733, 1:1000 dilution)(all Thermo Fisher Scientific).
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3

Immunofluorescence of E-cadherin

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The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (Thermo Fisher Scientific, #13-1900, 1:100 dilution) and Alexa Fluor 546-conjugated goat anti-rat IgG (H+L) antibody (Thermo Fisher Scientific, #A11081, 1:1000 dilution).
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