ERK1/2, phospho-ERK1/2 (Thr202/Tyr204), Akt (pan), phospho-Akt (S473), CD79b, CD79a, phospho-CD79a (Tyr182), SHP1, phospho-SHP1 (Tyr564), SHIP1, phospho-SHIP1 (Tyr1020), BTK, phospho-BTK(Tyr223), NF-kB p65, phospho–NF-kB p65 (ser536), IgM, Lyn, phospho-Lyn (Y507), LCK, CD86, and ZAP70 antibodies were from Cell Signaling Technology (Beverly, MA). Anti-SRC family (phospho-Y418)–phospho-Lyn (Y396), CD62L, and IgG antibodies were from Abcam (Cambridge, UK). Purified anti-human actin antibody was obtained from MP Biomedicals (Illkirch, France). Goat anti Rabbit IgG (H+L)–HRP conjugate and Goat anti Mouse IgG (H+L)–HRP conjugate, Goat F(ab′)2 anti-human IgM and Goat F(ab′)2 anti-human IgG were from Jackson Immunoresearch Laboratories (West Grove, PA). All antibodies utilized in the study were used in concentrations according to the manufacturer’s instructions. Ficoll-Paque PLUS from GE healthcare (Uppsala, Sweden), dimethyl sulfoxide (DMSO) from Merck (Darmstadt, Germany), RPMI, fetal calf serum (FCS), Dulbecco’s phosphate-buffered saline (PBS), L-glutamine, and penicillin-streptomycin from Biological Industries (Beit-Haemek, Israel) were utilized for cell cultures.
Phospho shp1 tyr564
Manufactured by Cell Signaling Technology
Sourced in Morocco
Phospho-SHP1 (Tyr564) is a primary antibody that recognizes SHP1 (also known as PTPN6) phosphorylated at tyrosine 564. SHP1 is a tyrosine phosphatase that plays a role in the regulation of cellular signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Zap70, by Cell Signaling Technology (1 mentions)
Nf kb p65, by Cell Signaling Technology (1 mentions)
Dulbecco s phosphate buffered saline, by Sartorius (1 mentions)
Penicillin streptomycin, by Sartorius (1 mentions)
Fetal calf serum (fcs), by Sartorius (1 mentions)
Roswell park memorial institute (rpmi), by Sartorius (1 mentions)
Cd79a, by Cell Signaling Technology (1 mentions)
F ab 2 goat anti human igm, by Jackson ImmunoResearch (1 mentions)
Purified anti human actin antibody, by MP Biomedicals (1 mentions)
Goat anti rabbit igg h l hrp conjugate and goat anti mouse igg h l hrp conjugate, by Jackson ImmunoResearch (1 mentions)
Dimethyl sulfoxide dmso from, by Merck Group (1 mentions)
L glutamine, by Sartorius (1 mentions)
Ficoll paque plus, by GE Healthcare (1 mentions)
C src, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
2 protocols using phospho shp1 tyr564
1
Multiparameter Immune Cell Analysis
2
Signaling Pathway Analysis of Immune Cells
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Whole-cell lysates of sorted non-plasmablast or plasmablast were separated by SDS–PAGE and blotted using standard procedures. The following antibodies were used: phospho-Shp1 (Tyr564), phospho-Src family kinase (Tyr416) (Cell Signaling), phospho-PI3-kinase p85α (Santa Cruz), c-Src (Cell Signaling) and PI3-kinase p85α (Upstate). The membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Santa Cruz), and visualized with SuperSignal West Pico/Dura chemiluminescent substrate (Pierce).
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