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Dynal cd4 cell isolation kit

Manufactured by Thermo Fisher Scientific
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The Dynal CD4 cell isolation kit is a laboratory product designed for the isolation and enrichment of human CD4+ T cells from peripheral blood mononuclear cells (PBMCs) or other sample types. The kit utilizes magnetic beads coated with anti-CD4 antibodies to selectively bind and isolate CD4+ cells from the sample.

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Lab products found in correlation

Histopaque 1083, by Merck Group (2 mentions)

Close spelling variants of this product

Dynal Mouse CD4 Cell Negative Isolation Kit (3 citations) Dynal CD4+ T cell isolation kit (2 citations) Dynal CD4+ T cell negative isolation kit (2 citations)

2 protocols using dynal cd4 cell isolation kit

1

Isolation and Culture of CD4+ T Cells

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Mononuclear cells were isolated from aseptically harvested ILN using Histopaque 1083 (Sigma-Aldrich, St Louis, MO) density gradient centrifugation and pooled from 2 or 3 mice to provide sufficient numbers of cells for culture. CD4+ T cells were purified through negative selection using a Dynal CD4 cell isolation kit (Invitrogen, Carlsbad, CA). Cells were cultured in 24-well culture plates at a density of 2 × 106 cells/ml in the presence of equal numbers of mitomycin C-inactivated spleen cells to serve as APC, either with no stimulus or with 2 × 107N. gonorrhoeae cells.
Liu Y., Hammer L.A., Liu W., Hobbs M.M., Zielke R.A., Sikora A.E., Jerse A.E., Egilmez N.K, & Russell M.W. (2017). Experimental Vaccine Induces Th1-driven Immune Responses and Resistance to Neisseria gonorrhoeae Infection in a Murine Model. Mucosal immunology, 10(6), 1594-1608.
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2

Isolation and Culture of CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mononuclear cells were isolated from aseptically harvested ILN using Histopaque 1083 (Sigma-Aldrich, St Louis, MO) density gradient centrifugation and pooled from 2 or 3 mice to provide sufficient numbers of cells for culture. CD4+ T cells were purified through negative selection using a Dynal CD4 cell isolation kit (Invitrogen, Carlsbad, CA). Cells were cultured in 24-well culture plates at a density of 2 × 106 cells/ml in the presence of equal numbers of mitomycin C-inactivated spleen cells to serve as APC, either with no stimulus or with 2 × 107N. gonorrhoeae cells.
Liu Y., Hammer L.A., Liu W., Hobbs M.M., Zielke R.A., Sikora A.E., Jerse A.E., Egilmez N.K, & Russell M.W. (2017). Experimental Vaccine Induces Th1-driven Immune Responses and Resistance to Neisseria gonorrhoeae Infection in a Murine Model. Mucosal immunology, 10(6), 1594-1608.
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