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Anti thiophosphate ester

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Anti-Thiophosphate ester is a laboratory reagent used to detect the presence of thiophosphate esters in samples. It provides a simple and reliable method for identifying the presence of these chemical compounds.

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Lab products found in correlation

Anti flag m2, by Merck Group (2 mentions) Rabbit polyclonal anti ha, by Abcam (1 mentions) Mouse monoclonal anti β actin, by Merck Group (1 mentions) P cdk1, by Cell Signaling Technology (1 mentions) Anti xpress, by Thermo Fisher Scientific (1 mentions) Anti c myc agarose affinity gel, by Merck Group (1 mentions) Anti c myc, by BioLegend (1 mentions) Gfp trap, by Proteintech (1 mentions) Anti ubiquitin, by Cell Signaling Technology (1 mentions) Streptavidin peroxidase, by Merck Group (1 mentions) Anti rps6, by Cell Signaling Technology (1 mentions) Anti phospho akt substrate, by Cell Signaling Technology (1 mentions) Anti gfp, by Roche (1 mentions) Anti rpb1, by Santa Cruz Biotechnology (1 mentions) Phos tag acrylamide, by Fujifilm (1 mentions) β actin, by Merck Group (1 mentions) Anti β tubulin, by Santa Cruz Biotechnology (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Lambda phosphatase, by Merck Group (1 mentions)

Spelling variants of this product

Anti-thiophosphate ester antibody (9 citations) Thiophosphate ester (3 citations) Recombinant Anti-Thiophosphate ester antibody (2 citations)

3 protocols using anti thiophosphate ester

1

Western Blot and Co-IP Analysis of TAZ Signaling

Cited 1 time
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Protein extract, western blot and co-IP were performed as described [36 (link)]. Antibodies used were as followings: mouse monoclonal anti-TAZ (1:1000) antibody from BD Biosciences; mouse monoclonal anti-β-actin (1:10, 000) and anti-FLAG (M2; 1:500) antibodies from Sigma; rabbit polyclonal anti-HA (1:1000) and anti-Thiophosphate ester (1:1000) antibodies from Abcam; rabbit polyclonal anti-MST1, MST2, LATS1, CyclinB1, Cdk1, cleaved-PARP (c-PARP), BCL-2, and pCdk1 (all diluted at 1:1000) antibodies from Cell Signalling Technology.
Zhao Y, & Yang X. (2015). Regulation of sensitivity of tumor cells to antitubulin drugs by Cdk1-TAZ signalling. Oncotarget, 6(26), 21906-21917.
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2

Immunoprecipitation and Western Blotting Protocols

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The immunoprecipitation experiments were performed as previously described19 (link). Anti-FLAG M2 affinity gel (Sigma-Aldrich), anti-GFP agarose beads (GFP-Trap, Chromotek) or Anti-c-MYC agarose affinity gel (Sigma-Aldrich) were used for immunoprecipitation. The sample preparation for the western blotting was performed using an alkaline-protein30 (link) or TCA18 (link) extraction method. Anti-c-MYC (1:1,500 dilution; BioLegend), anti-GFP (1:1,500; Roche), Anti-FLAG M2 (1:1,500; Sigma-Aldrich), anti-ubiquitin (1:1,500; Cell Signaling Technology), anti-thiophosphate-ester (1:1,500, Abcam), anti-Xpress (1:1,500; Thermo Fisher), anti-phospho-Akt substrate (1:1,500; Cell Signaling Technology), anti-RPS6 (1:1,500; Cell Signaling Technology) antibodies and streptavidin-peroxidase (1:5,000; Sigma-Aldrich) were used for probing.
Wei Y., Lee N.N., Pan L., Dhakshnamoorthy J., Sun L.L., Zofall M., Wheeler D, & Grewal S.I. (2021). TOR targets an RNA processing network to regulate facultative heterochromatin, developmental gene expression and cell proliferation. Nature cell biology, 23(3), 243-256.
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3

Phosphorylation-Dependent Protein Detection

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Primary antibodies included: anti-CDC7 (MBL International), anti-TOP2A (Millipore), anti- NWSHPQFEK/Strep-tag (GenScript), anti-centromere antibodies (ACA) (Antibodies Incorporated), anti-MCM2 (AbD Serotec), anti-pSer40/41 MCM2 described in (7 (link)), β-actin (Sigma), anti-RPB1 (Santa-Cruz Biotechnology), anti-β-tubulin (Santa-Cruz Biotechnology), and anti-thiophosphate ester (Abcam). Equal amounts of cell extract were resolved on 7.5% or 10% gels for standard SDS-PAGE, or 5% gels with 5 μM Phos-tag acrylamide (Wako Pure Chemical Industries) and 10 μM MnCl2 for phosphorylation-dependent mobility shift detection, and subject to western blot analysis. IRDye® secondary antibodies for protein detection using the Odyssey infrared imaging system were used (Li-COR Biosciences). For dephosphorylation assays, 20 μg of extracts were incubated with 20 U of Lambda Phosphatase (Sigma) for 30 min before SDS-PAGE.
Wu K.Z., Wang G.N., Fitzgerald J., Quachthithu H., Rainey M.D., Cattaneo A., Bachi A, & Santocanale C. (2016). DDK dependent regulation of TOP2A at centromeres revealed by a chemical genetics approach. Nucleic Acids Research, 44(18), 8786-8798.
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