BEAS-2B cells were stained with primary antibody for γ-H2AX (Epitomics) and slides were incubated for 1 h with Alexa-488-conjugated anti-rabbit IgG for visualization of foci. Luorescence analyses were performed with a DM 6000 B microscope (Leica, Wetzlar, Germany). Every microscope slide was counted at least three times by two blinded observers independently. In order to get the x-ray induced γ-H2AX-foci (so-called excess γ-H2AX-foci) we subtracted the absolute γ-H2AX-foci before irradiation (so-called background foci) from the absolute γ-H2AX-foci after exposure.
Primary antibody for γ h2ax
Manufactured by Abcam
Sourced in United States
The primary antibody for γ-H2AX is a laboratory reagent used to detect the phosphorylation of the histone variant H2AX, which is a marker of DNA double-strand breaks. This antibody can be used in various techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the cellular response to DNA damage.
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Lab products found in correlation
2 protocols using primary antibody for γ h2ax
1
Quantification of DNA Damage Foci
2
Immunofluorescence Assay for γH2AX Foci
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Slides were stained with primary antibody for γH2AX (Epitomics, CA, USA) and incubated with Alexa-488-conjugated anti-rabbit IgG. Visualization of foci was performed by a FV1200 confocal microscope (Olympus, Japan). Every slide was counted at least 3 times by two blinded observers independently.
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