Safranin O/Fast green staining was used to assess glycosaminoglycans (GAGs) content. Samples were fixed in 10% buffered formalin phosphate solution (Fisher Chemical, Fair Lawn, NJ, United States) overnight and dehydrated with an increasing ethanol gradient. After being soaked in xylene for 2 h and then in liquid paraffin overnight, samples were embedded in paraffin. The blocks were sectioned at 6 μm thickness using the RM 2255 Fully Automated Rotary Microtome (Leica Biosystems, Buffalo Grove, IL, United States). Slides were dried and subjected to Safranin O/Fast green counterstaining (Sigma-Aldrich, St. Louis, MO, United States). Cell nuclei were stained with Hematoxylin solution (Sigma-Aldrich).
Buffered formalin phosphate solution
Manufactured by Thermo Fisher Scientific
Sourced in United States
Buffered formalin phosphate solution is a fixative used in histology and microscopy to preserve biological samples. It contains a combination of formaldehyde and phosphate buffer to maintain a stable pH. This solution is commonly used to prepare tissue samples for further analysis and examination.
Automatically generated - may contain errors
3 protocols using buffered formalin phosphate solution
1
Safranin O/Fast Green Staining for GAGs
2
Synergistic Apoptosis Induction
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Cells were seeded in a 96-well plate with 1 x 104 cells per well in 100 μL of medium. On the following day, cells were treated with combinations of Ad-NOXA or fenretinide with ABT-263. After 72 hours, cells were fixed with 10% Buffered formalin phosphate solution (Fisher Scientific, Hampton, NH) and then stained with 0.05% crystal violet gram stain (Fisher Scientific).
3
Histological Analysis of Mouse Lungs
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Lungs were removed from euthanized mice and submerged in a 10% buffered formalin phosphate solution (Fisher Scientific). Tissues were placed in fresh formalin 24 to 72 h postfixation and then submitted to the University of Colorado Denver Cancer Center histology facility. Tissues were paraffin embedded, and tissue sections were stained with hematoxylin and eosin prior to analysis by a pathologist (C.D.C.).
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