Pathogen-free, juvenile, male (C57BL/6J) wild type (WT) and NLRP3 knock out (NLRP3 KO) mice were purchased from the Jackson Laboratories (Bar Harbor, ME) and were used for the study.
Nlrp3 ko
Manufactured by Jackson ImmunoResearch
NLRP3 KO is a laboratory product that allows for the genetic knockout of the NLRP3 gene. It can be used to study the role of the NLRP3 inflammasome in various biological processes.
Automatically generated - may contain errors
4 protocols using nlrp3 ko
1
NLRP3 Knockout Mouse Model
2
Investigating Btk and NLRP3 Knockouts
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Btk KO (originally generated by Khan et al., 1995 (link)), NLRP3 KO (stock no. 021302; The Jackson Laboratory), and WT mice, all on a C57BL/6J (The Jackson Laboratory) background, were maintained locally in specific pathogen–free conditions under regular hygiene monitoring. All animal experiments were approved by local authorities and performed in accordance with local institutional guidelines and animal protection laws, including specific locally approved protocols for sacrificing.
3
Genetically Engineered Mouse Models
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Wild-type (WT) C57BL/6 J (JAX, Strain# 000664), Nlrp3 KO (JAX, Strain# 021302), and Rag1 KO mice (JAX, Strain# 002216), both on the C57BL/6 J background, were originally purchased from the Jackson Laboratory (JAX). Initial breeding pairs of Il1b KO mice (JAX, Strain# 034447) backcrossed to C57BL/6 J for over 10 generations were kindly provided by Dr. Wai Wilson Cheung, Dr. Robert Mak, and Dr. Hal Hoffman (UCSD). Initial breeding pairs of Casp1 KO mice (JAX, Strain# 016621) on the C57BL/6 background were a gift from Dr. Richard Flavell (Yale University). Initial breeding pairs of CCSPCre (JAX, Strain# 036525) [29 (link)] and LSL-KrasG12D (JAX, Strain# 008179) [30 (link)] mice, both on the C57BL/6 background, were a gift from Dr. Seon Hee Chang (The University of Texas MD Anderson Cancer Center). For the generation of KrasG12D mice, CCSPCre± mice were intercrossed with LSL-KrasG12D± as detailed in the KrasG12D-driven LC model section. All the mice were bred in our vivarium under specific pathogen-free (SPF) or enhanced-barrier SPF (Rag1 KO) conditions for more than 6 months and were genotyped before they were used in any experiments. All the mice were kept on a 12-h light and 12-h dark cycle with a standard chow diet and water.
4
Septic Neuroinflammation Mouse Model
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The septic neuroinflammation mouse model for optimal NET generation was induced by an intravenous injection of 2.5 mg/kg Escherichia coli-derived LPS (O111:B4, Sigma-Aldrich, St. Louis, MO, USA) for 24 h. C57BL/6 mice (Orient Bio, Seongnam, Korea) and NLRP3 KO (Jackson Laboratory, Farmington, CT, USA) were maintained in a specific pathogen-free environment at Avison Biomedical Research Center at the Yonsei University College of Medicine. All animal experiments were approved by the Institutional Animal Care and Use Committee of the Yonsei University College of Medicine (IACUC No. 2019–0097).
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