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Pe conjugated anti iga antibody

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The PE-conjugated anti-IgA antibody is a laboratory reagent used to detect and quantify IgA antibodies in biological samples. It consists of a phycoerythrin (PE) fluorescent dye conjugated to an antibody specific for the IgA antibody.

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Lab products found in correlation

Facscanto 2 flow cytometer, by BD (1 mentions) Tgf β1, by Thermo Fisher Scientific (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Anti cd40, by BD (1 mentions) Cytoflex flow cytometer, by Beckman Coulter (1 mentions)

Spelling variants of this product

IgA-PE (4 citations) PE-conjugated anti-murine IgA antibody (clone 11–44-2, (2 citations)

2 protocols using pe conjugated anti iga antibody

1

Inducing Class Switching in CH12F3-2 Cells

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To induce class switching, 200,000 transduced CH12F3-2 cells were seeded into 24-well plate and treated with 1 ng/ml TGF-β1 (Preprotech), 10 ng/ml IL-4 (Preprotech) and 1 μg/ml agonist anti-CD40 (BD). Cells were stained with a PE-conjugated anti-IgA antibody (eBiosciences) and analyzed using a FACSCantoII flow cytometer (BD Biosciences) and Flowing Software.
Dulev S., Tkach J., Lin S, & Batada N.N. (2014). SET8 methyltransferase activity during the DNA double-strand break response is required for recruitment of 53BP1. EMBO Reports, 15(11), 1163-1174.
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2

Quantifying IgA Switching Efficiency

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For detecting IgA switching efficiency, 1 3 10 6 cells were stained with 0.1 mg of phycoerythrin (PE)-conjugated anti-IgA antibody (eBioscience, 12-4204-83) and 0.1 mg of APC-conjugated anti-IgM antibody (eBioscience, 17-5790-82) for 5 min at room temperature in 200 mL of 2% FBS/PBS. After washing twice with PBS, cells were adjusted to a concentration of 1-5 3 10 6 cells/mL and were analyzed on a CytoFLEX Flow Cytometer (Beckman Coulter). Flow cytometry data were analyzed using CytExpert software or FlowJo software. Live cells were gated on the FSC/SSC plot and IgA+ cells were further identified on the IgA PE/IgM APC plot.
Bai W., Zhu G., Xu J., Chen P., Meng F., Xue H., Chen C, & Dong J. (2021). The 3'-flap endonuclease XPF-ERCC1 promotes alternative end joining and chromosomal translocation during B cell class switching. Cell reports, 36(13).
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