LHC-9 cell culture medium, 5-(and 6-) chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA in short H2DCFDA), dihydroethidium (DHE) were from Life Technologies (Carlsbad, CA, USA). Sterile HBS and purified collagen, PureCol™, were from Inamed Biomaterials (Freemont, CA, USA). 1,3-Dinitropyrene (1,3-DNP), 1,8-dinitropyrene (1,8-DNP), benzo[a]pyrene (B[a]P), bovine serum albumin (BSA), dimethyl sulfoxide (DMSO), ethylenediamineteraacetic acid (EDTA), Hoechst 33258, Hoechst 33342, aprotinin, Pifithrin-μ (PFT-μ), Ponceau S, phenylmethylsulfonyl fluoride (PMSF), propidium iodide (PI), polyoxyethylene octyl phenyl ether (Triton X-100) and zVAD-FMK were obtained from Sigma–Aldrich (St. Louis, MO, USA). Pifithrin-α (PFT-α) and Pepstatin A were from Calbiochem (Cambridge, CA, USA). Leupeptin was from Amersham Biosciences (Uppsala, Sweden). Bio-Rad DC protein assay was from Bio-Rad Laboratories, Inc (Hercules, CA, USA). Fetal calf serum (FCS), gentamycin, MEM alpha medium with l -glutamine, without ribonucleosides and deoxyribonucleosides were from Gibco BRL (Paisley, UK). UltraPure™ Low Melting-Point Agarose was purchased from Invitrogen (Paisley, UK). All other chemicals were of analytical grade and purchased from commercial sources.
Leupeptin
Manufactured by Cytiva
Sourced in Sweden, United States
Leupeptin is a protease inhibitor commonly used in laboratory settings. It functions by inhibiting the activity of certain proteases, which are enzymes that break down proteins. Leupeptin is often employed in research applications to preserve protein integrity and prevent unwanted proteolysis.
Automatically generated - may contain errors
Lab products found in correlation
Dihydroethidium dhe, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Aprotinin, by Merck Group (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
Ultrapure low melting point agarose, by Thermo Fisher Scientific (1 mentions)
Z vad fmk, by Merck Group (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Ponceau s, by Merck Group (1 mentions)
Dc protein assay, by Bio-Rad (1 mentions)
Pifithrin μ pft μ, by Merck Group (1 mentions)
Brefeldin a, by Merck Group (1 mentions)
Pepstatin, by Roche (1 mentions)
2 protocols using leupeptin
1
Cellular Oxidative Stress Evaluation
2
Protease Inhibitors for Cellular Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Brefeldin A (BFA) and all protease inhibitors were purchased from Sigma-Aldrich (Saint Louis, MO, USA), with the exception of lactacystin (from Dr. E.J. Corey, Harvard University, Cambridge, MA, USA), leupeptin (Amersham, Little Chalfont, Bucks., UK), pepstatin (Boehringer Mannheim, Mannheim, Germany), and Z-VAD-FMK (Enzyme System Products, Livermore, CA, USA). The specificity of inhibitors used is summarized inTable 1 .
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