αLy6c (clone Monts1, BE0203, BioXcell, USA) Isotype control (clone 2A3, BE0089, BioXcell, USA).
αLy6C (clone HK1.4, 128022, BioLegend, USA).
αLy6C (clone HK1.4, 128022, BioLegend, USA).
αly6c clone hk1
Manufactured by BioLegend
Sourced in United States, Canada
αLy6C (clone HK1.4) is a monoclonal antibody that recognizes the Ly6C antigen. Ly6C is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein expressed on various cell types, including monocytes, macrophages, and a subset of T cells. This antibody can be used for the identification and analysis of Ly6C-positive cells in flow cytometry and other immunological applications.
Automatically generated - may contain errors
2 protocols using αly6c clone hk1
1
Ly6C Antibody and Isotype Control
2
Multicolor flow cytometry analysis
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Tumor singlets and MGFP suspensions in FACS buffer were treated with α-CD16/32 (clone 93, 1:200; BioLegend) at 4 °C for 20 min to block Fc receptors, followed by incubation with fluorescent-labeled antibodies at 4 °C in the dark for 25 min in FACS buffer. The following antibodies were used: α-CD11b (clone M1/70, 1:100; BioLegend), α-F4/80 (clone CI:A3-1, 1:20; Bio-Rad Laboratories) for MGTRMs, α-CD11c (clone N418, 1:100; eBioscience, San diego, CA), α-MHC-II (clone M5/114.15.2, 1:100, BD Bioscience) for dendritic cells, α-CD11b (1:100), α-Ly-6c (clone HK1.4, 1:200; BioLegend) for tumor-infiltrating monocytes, α-CD8a (clone 53-6.7, 1:100; eBioscience) for T-cells, and α-NK1.1 (clone PK136, 1:100; eBioscience) for natural killer cells. The antibody-labeled cells were sorted and analyzed using an SH800Z fluorescence activated cell sorter and EC800 flow cytometry analyzer (Sony, Tokyo, Japan), respectively.
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