Western blot was performed as described previously (Fujiwara et al., 2021 (link)). Total protein from ALC cells treated with/without PFOA were extracted and equal amounts of protein were subjected to Immunoblot analysis. The following antibodies were used. Primary antibodies: rabbit anti-cleaved caspase 3, rabbit anti-γH2AX, rabbit anti-phospho-ERK, rabbit anti-total ERK antibodies (Cell Signaling Technology, Boston, MA, USA). Mouse anti-β-actin (Cell Signaling Technology) was used as a loading control protein. Secondary antibodies: HRP-conjugated anti--rabbit or mouse IgG secondary antibodies (Cell Signaling Technology). Signal was detected using the ChemiDoc™ Imaging System (Bio-Rad). Band densities were quantified by Image Lab™ software (Bio-Rad). Representative images are shown in the results section. Data of relative protein level are presented as means ± SD. Each experiment was performed in triplicate.
Rabbit anti total erk antibodies
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit anti-total ERK antibodies are primary antibodies that recognize the extracellular signal-regulated kinases (ERK) proteins, which are important components of the mitogen-activated protein kinase (MAPK) signaling pathway. These antibodies can be used to detect and quantify the total amount of ERK proteins in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti β actin, by Cell Signaling Technology (2 mentions)
Rabbit anti γh2ax, by Cell Signaling Technology (2 mentions)
Rabbit anti phospho erk, by Cell Signaling Technology (2 mentions)
Hrp conjugated anti rabbit or mouse igg secondary antibodies, by Cell Signaling Technology (2 mentions)
Image lab software, by Bio-Rad (2 mentions)
Chemidoc imaging system, by Bio-Rad (2 mentions)
2 protocols using rabbit anti total erk antibodies
1
Quantitative Western Blot Analysis of Protein Expression
2
Quantitative Western Blot Analysis of Protein Expression
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Western blot was performed as described previously (Fujiwara et al., 2021 (link)). Total protein from ALC cells treated with/without PFOA were extracted and equal amounts of protein were subjected to Immunoblot analysis. The following antibodies were used. Primary antibodies: rabbit anti-cleaved caspase 3, rabbit anti-γH2AX, rabbit anti-phospho-ERK, rabbit anti-total ERK antibodies (Cell Signaling Technology, Boston, MA, USA). Mouse anti-β-actin (Cell Signaling Technology) was used as a loading control protein. Secondary antibodies: HRP-conjugated anti--rabbit or mouse IgG secondary antibodies (Cell Signaling Technology). Signal was detected using the ChemiDoc™ Imaging System (Bio-Rad). Band densities were quantified by Image Lab™ software (Bio-Rad). Representative images are shown in the results section. Data of relative protein level are presented as means ± SD. Each experiment was performed in triplicate.
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