Ifnγ 285 if 100

Manufactured by R&D Systems

Sourced in United States

IFNγ (285-IF-100) is a recombinant human interferon gamma protein product manufactured by R&D Systems. It has a molecular weight of approximately 17 kDa.

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Lab products found in correlation

Cd68 antibody ab125212, by Abcam (1 mentions) Poly da dt lyovec, by InvivoGen (1 mentions) 2 3 cgamp, by InvivoGen (1 mentions)

Spelling variants of this product

Human IFNγ (285-IF-100) (2 citations)

2 protocols using ifnγ 285 if 100

Multiparametric Immune Profiling

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CD3 antibody (clone F7.2.38; MA5-12577) and CD45RO antibody (clone UCHL1; MA5-11532) were from Thermo Scientific. CD4 antibody (clone 4B12; MS1528S0) and CD8 antibody (clone C8/144B; MS457S0) were from Fisher Scientific. CD68 antibody (ab125212), CD4 antibody (ab133616) and FOXP3 antibody (clone 236A/E7; ab20034) were from Abcam. PD-L1 antibody (E1L3N; 13684) and p-STAT3 (Y701; 9167) were from Cell Signaling Technologies. GAPDH antibody (FL-335; sc25778) was from Santa Cruz Biotechnology. IFNγ (285-IF-100) was from R&D systems.

Liu X.D., Hoang A., Zhou L., Kalra S., Yetil A., Sun M., Ding Z., Zhang X., Bai S., German P., Tamboli P., Rao P., Karam J.A., Wood C., Matin S., Zurita A., Bex A., Griffioen A.W., Gao J., Sharma P., Tannir N., Sircar K, & Jonasch E. (2015). Resistance to anti-angiogenic therapy is associated with an immunosuppressive tumor microenvironment in metastatic renal cell carcinoma. Cancer immunology research, 3(9), 1017-1029.

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Interferon Signaling Pathway Activation

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Doxycycline hyclate (D9891-1G) was purchased from Sigma-Aldrich (Taufkirchen, Germany) and used at a concentration of 0.5 μg/ml. Recombinant human IFN-β (#11415–1) and IFN-γ (#285-IF-100) were purchased from R&D systems (Minneapolis, MN, USA). Poly(dA:dT)/LyoVec (#tlrl-patc), poly(I:C) high molecular weight (HMW)/LyoVec (#tlrl-piclv), poly(I:C) low molecular weight (LMW)/LyoVec (#tlrl-picwlv) and 2’3’-cGAMP (#tlrl-nacga23) were purchased from InvivoGen (Toulouse, France). Ruxolitinib (#AG-CR1-3624) was purchased from Biomol GmbH (Hamburg, Germany). Stock solutions were prepared and substances were either used directly or stored according to the manufacturers’ instructions.

Stilp A.C., Scherer M., König P., Fürstberger A., Kestler H.A, & Stamminger T. (2022). The chromatin remodeling protein ATRX positively regulates IRF3-dependent type I interferon production and interferon-induced gene expression. PLoS Pathogens, 18(8), e1010748.

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