After 3-h adherence on RGD-membrane, cells were treated firstly with 4% paraformaldehyde and then 0.1% Triton X-100 treatment for 20 min each at 37 °C. 5% BSA with 10% normal donkey serum (blocking buffer) was used for overnight blocking at 4 °C. Samples were then incubated with the primary antibody in blocking buffer at 4 °C for 48 h. Primary antibody was then washed with PBS, followed by secondary antibody incubation for 2 h in room temperature. Primary antibodies included activated human integrin-β3 antibody (1:25, anti-LIBS2 epitope clone ab62, Millipore MABT27) and pFAK-Y397 (1:50, Thermo Fisher Scientific 44-624G). Secondary antibodies included AF594-anti-mouse (1:1000, Thermo Fisher Scientific A-21203) and AF594-anti-rabbit (1:1000, Thermo Fisher Scientific A-21207).
Af594 anti mouse
Manufactured by Thermo Fisher Scientific
AF594-anti-mouse is a fluorescent-labeled secondary antibody that binds to mouse primary antibodies. It is used for the detection and visualization of mouse-derived targets in various immunoassays and microscopy applications.
Automatically generated - may contain errors
Lab products found in correlation
Af594 anti rabbit, by Thermo Fisher Scientific (2 mentions)
A21207, by Thermo Fisher Scientific (1 mentions)
Pfaky397, by Thermo Fisher Scientific (1 mentions)
A21203, by Thermo Fisher Scientific (1 mentions)
Anti rabbit af488, by Thermo Fisher Scientific (1 mentions)
Sc 516102, by Santa Cruz Biotechnology (1 mentions)
2 protocols using af594 anti mouse
1
Integrin-β3 and pFAK-Y397 Immunostaining
2
Integrin and Src Signaling Pathway
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary antibodies included anti-pY416Src (CST 2101, 1:1,000 for Western blot [WB]), anti-Src (CST 2109, 1:1,000 for WB), anti-PIK3CA (Abcam ab40776; 1:100 for immunofluorescence [IF], 1:1,000 for WB), SHIP2 (Abcam ab70267; 1:100 for IF, 1:1,000 for WB), anti-AKTIP (Atlas Antibodies HPA046300; 1:1,000 for WB), anti-SNX9 (Atlas Antibodies HPA031410; 1:1,000 for WB), activated human integrin-β3 antibody (Millipore MABT27, LIBS2, epitope clone ab62; 1:50 for IF), anti-pFAK-Y397 (Thermo Fisher Scientific 44-624G; 1:100 for IF, 1:1,000 for WB), anti-FAK (Abcam ab40794; 1:1,000 for WB), anti-tubulin (CST 2146; 1:10000 for WB), and anti-GAPDH (Thermo Fisher Scientific AM4300; 1:10,000 for WB). Secondary antibodies included AF594-anti-mouse (Thermo Fisher Scientific A-21203; 1:1,000 for IF), AF594-anti-rabbit (Thermo Fisher Scientific A-21207; 1:1,000 for IF), AF488-anti-mouse (Thermo Fisher Scientific A-21202; 1:1,000 for IF) and AF488-anti-rabbit (Thermo Fisher Scientific A-21206; 1:1,000 for IF), anti-mouse horseradish peroxidase (HRP) (Santa Cruz sc-516102; 1:2,000 for WB), and anti-rabbit HRP (CST 7074; 1:2,000 for WB).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!