DIF microscopy was performed with MONOFLUO Pneumocystis jirovecii IFA test kit number 32515 (Bio-Rad). Lack of positive controls from “definite” PCP patients was a challenge during the study period. For this reason and concerns regarding sensitivity and specificity, the laboratory used DIF as a complementary method in line with the guidelines (7 (link)), mainly on PCR-positive samples. In 2017, semiquantitative real-time PCR replaced DIF definitely. The in-house assay targeting the beta-tubulin gene of P. jirovecii was adapted from Brancart et al. (33 (link)) with some modifications as described in detail below (11 (link), 33 (link)).
Monofluo pneumocystis jirovecii ifa test kit number 32515
Manufactured by Bio-Rad
The MONOFLUO Pneumocystis jirovecii IFA test kit number 32515 is a laboratory equipment product designed for the detection of Pneumocystis jirovecii, a fungus that can cause pneumonia in immunocompromised individuals. The kit utilizes an indirect immunofluorescence assay (IFA) method to detect the presence of the Pneumocystis jirovecii organism in clinical samples.
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2 protocols using monofluo pneumocystis jirovecii ifa test kit number 32515
1
Pneumocystis jirovecii Detection by DIF and Real-Time PCR
2
Pneumocystis jirovecii Detection by DIF and Real-Time PCR
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DIF microscopy was performed with MONOFLUO Pneumocystis jirovecii IFA test kit number 32515 (Bio-Rad). Lack of positive controls from “definite” PCP patients was a challenge during the study period. For this reason and concerns regarding sensitivity and specificity, the laboratory used DIF as a complementary method in line with the guidelines (7 (link)), mainly on PCR-positive samples. In 2017, semiquantitative real-time PCR replaced DIF definitely. The in-house assay targeting the beta-tubulin gene of P. jirovecii was adapted from Brancart et al. (33 (link)) with some modifications as described in detail below (11 (link), 33 (link)).
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