D-(+)-Glucose, glucose oxidase (GOx) from Aspergillus niger, 4-aminoantipyrine (4-AAP), albumin from human serum, tetrabromophenol blue (TBPB), citric acid, cholesterol (Chol.) ALP, alkaline phosphatase yellow (p-nitrophenyl phosphate), an AST activity assay kit, an alanine aminotransferase (ALT) activity assay kit, a creatinine assay kit, and ethyl alcohol were purchased from Sigma-Aldrich Korea (Seoul, Korea). A urea nitrogen (UN) colorimetric detection kit was purchased from Thermo Fisher Scientific (San Jose, CA, USA). Horseradish peroxidase (HRP), cholesterol oxidase (CO), and cholesterol esterase (CE) were obtained from Toyobo Co. (Osaka, Japan). N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate (MAOS), N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methoxyaniline, sodium salt, and dihydrate (ADOS) were purchased from Dojindo (Rockville, MD, USA).
Tetrabromophenol blue tbpb
Manufactured by Merck Group
Sourced in Japan
Tetrabromophenol blue (TBPB) is a chemical compound commonly used as a pH indicator in laboratory settings. It is a blue-colored dye that changes color depending on the pH of the solution it is added to, making it a useful tool for measuring and monitoring pH levels in various applications.
Automatically generated - may contain errors
Lab products found in correlation
N ethyl n 2 hydroxy 3 sulfopropyl 3 5 dimethylaniline sodium salt monohydrate maos, by Dojindo Laboratories (1 mentions)
Alanine aminotransferase alt activity assay kit, by Merck Group (1 mentions)
D glucose, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Ast activity assay kit, by Merck Group (1 mentions)
Creatinine assay kit, by Merck Group (1 mentions)
Glucose oxidase gox from aspergillus niger, by Merck Group (1 mentions)
Trisodium citrate, by Fujifilm (1 mentions)
2 protocols using tetrabromophenol blue tbpb
1
Biochemical Assay Protocol Compendium
2
Colorimetric Protein Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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BSA standard solution was purchased from Takara-Bio Co., Inc. (Shiga, Japan). Tetrabromophenol blue (TBPB) was purchased from Sigma-Aldrich Co., Inc. Citric acid was purchased from Hidex Co, Inc. (Osaka, Japan) and trisodium citrate was purchased from Wako Pure Chemical Industries, Ltd. BSA standard solution was diluted with ultrapure water to achieve the desired concentrations (0, 2, 4, 6, 8, 10, and 20 µM). For the protein assay, 15 µL of a 250 mM citrate buffer solution ( pH 1.8) was introduced into the auxiliary zone and exposed to air at room temperature for 10 min. Then, a 9 mM solution (15 µL) of TBPB in 95% ethanol was introduced into the citrate buffer solution residue followed by exposing to air for another 10 min. Finally, 0.5 µL of the different concentrations of BSA solutions were separately spotted onto eight sample zones.
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