Samples were lysed using radioimmunoprecipitation assay buffer (RIPA) lysis buffer (150 mM NaCl, 5 mM EDTA, 50 mM Tris pH 8.0, 0.5% sodium deoxycholate, 1% NP-40, 0.1% SDS) supplemented with 1 mM dithiothreitol and protease inhibitors. The lysates were separated by SDS-PAGE and analyzed using standard western blotting procedures (Khan et al., 2019 (link)). Antibodies: human NR4A1 (ab153914, 1:1,000; Abcam), mouse NR4A1 (14-5965-82, 1:1,000; Invitrogen), NR4A2 (AV38753, 1:1,000; Sigma-Aldrich), NR4A3 (TA804893, 1: 1,000; Thermo Fisher Scientific), VHL (68547, 1:1,000; Cell Signaling), β-actin (13E5, 1:5,000; Cell Signaling), β-tubulin (9F3, 1: 5,000; Cell Signaling), and GAPDH (D16H11, 1: 5,000; Cell Signaling) were employed for protein detection.
Ta804893
Manufactured by Thermo Fisher Scientific
The TA804893 is a laboratory instrument designed for thermal analysis. It is capable of measuring and analyzing the thermal properties of various materials. The core function of this product is to provide accurate and reliable data on the thermal behavior of samples, which can be useful in a wide range of scientific and industrial applications.
Automatically generated - may contain errors
2 protocols using ta804893
1
Protein Extraction and Detection Protocol
2
Western Blot Analysis of NR4A Receptors
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Samples were lysed using RIPA lysis buffer (150 mM NaCl, 5 mM EDTA, 50 mM Tris pH 8.0, 1% sodium deoxycholate, 1% NP-40, 0.5% SDS) supplemented with 1 mM dithiothreitol and protease inhibitors. The lysates were separated by SDS-PAGE and analyzed using standard western blotting procedures58 . Antibodies: human NR4A1 (ab153914, Abcam, 1:1000), mouse NR4A1 (14-5965-82, Invitrogen, 1:1000), NR4A2 (AV38753, Sigma, 1:1000), NR4A3 (TA804893, Thermo Fisher, 1: 1000), VHL (68547,Cell Signaling, 1:1000), β-actin (13E5, Cell Signaling, 1:5,000), β-tubulin (9F3, Cell Signaling, 1: 5000), GAPDH (D16H11, Cell Signaling, 1: 5000) were employed for protein detection.
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