Immunohistochemistry analyses were performed as described previously 17 (link). For apoptotic cells analysis, paraffin-embedded tissue sections were stained with anti-Perilipin (Abcam, Cat. No. ab-61682) and cleaved caspase-3 (Cell Signaling Technology, Cat No. 9664) antibodies. Images were captured using a NanoZoomer slide scanner system with NanoZoomer Digital Pathology software (Hamamatsu) or confocal fluorescence microscopy. Microscopic images were analyzed using ImageJ software.
Nanozoomer slide scanner system
Manufactured by Hamamatsu Photonics
Sourced in France
The NanoZoomer slide scanner system is a high-performance digital slide scanning solution designed for a wide range of applications. The system efficiently digitizes microscope slides, capturing high-resolution images with exceptional image quality. The NanoZoomer system is a versatile tool for various fields, including pathology, research, and education.
Automatically generated - may contain errors
3 protocols using nanozoomer slide scanner system
1
Apoptotic Cell Quantification in Tissue Sections
2
Quantification of Lung Metastases
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Lung metastasis analyses were performed using 3−μm−thin sections from formalin−fixed paraffin−embedded tissue blocks. Counterstaining was performed using Flex Hematoxylin (Dako−Agilent, Les Ulis, France) followed by washing the slides under tap water for 5 min. Finally, slides were mounted with a coverslip after dehydration. The NanoZoomer slide scanner system (Hamamatsu Photonics, Massy, France) was used to digitalize glass slides at the ×40 objective. The number of metastases was counted and adjusted to the total surface of each lung slide.
3
Apoptotic Cell Quantification in Tissue Sections
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