Alexa fluor 488 streptavidin antibody

Manufactured by Thermo Fisher Scientific

Sourced in United States

Alexa Fluor 488 streptavidin antibody is a fluorescently labeled reagent used in various immunoassay and microscopy applications. Streptavidin, a protein derived from the bacterium Streptomyces, binds strongly to the small molecule biotin. The Alexa Fluor 488 dye is attached to the streptavidin, providing a fluorescent signal that can be detected and measured.

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Lab products found in correlation

Rhodamine conjugated anti digoxigenin antibody, by Roche (2 mentions) Digoxigenin 11 deoxy uridine triphosphate, by Roche (1 mentions) Biotin 16 dutp, by Roche (1 mentions) Vectashield antifade solution, by Vector Laboratories (1 mentions) Axioplan 2 microscope, by Zeiss (1 mentions) Biotin 11 dutp, by Roche (1 mentions)

3 protocols using alexa fluor 488 streptavidin antibody

Comparative Chromosome Mapping in Cucurbitaceae

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To examine and validate chromosome rearrangements between cucumber C7, melon I, watermelon W2 and W9, 14 fosmid probes identified on cucumber C7 [12 (link)] were used in comparative FISH mapping of meiotic pachytene chromosomes prepared from pollen mother cells of melon and watermelon. The physical order of adjacent fosmid clones in each chromosome was determined by two-color FISH. The FISH procedure was performed as described by Koo et al. [63 (link)]. Biotin- and digoxigenin-labeled probes were detected with Alexa Fluor 488 streptavidin antibody (Invitrogen, Carlsbad, CA) and rhodamine-conjugated anti-digoxigenin antibody (Roche Diagnostics USA, Indianapolis, IN), respectively. Chromosomes were counterstained by 4¢, 6-diamidino-2-phenylindole (DAPI) in ‘Vector Shield’ antifade solution (Vector Laboratories, Burlingame, CA). FISH signals were captured using a CCD camera. The images were processed using Meta Imaging Series 7.5 software (Molecular Devices, Downingtown, PA, USA).

Zhu H., Song P., Koo D.H., Guo L., Li Y., Sun S., Weng Y, & Yang L. (2016). Genome wide characterization of simple sequence repeats in watermelon genome and their application in comparative mapping and genetic diversity analysis. BMC Genomics, 17, 557.

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Cloning and Labeling of EPSPS Gene

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Sequences of A. palmeri EPSPS gene (GenBank accession no. JX564536) were used to develop the PCR primers for cloning of the EPSPS gene. The PCR product was cloned in 2.1-TOPO TA vector (Invitrogen), and the clone was labeled with digoxigenin-11-deoxyuridine triphosphate (Roche Diagnostics) using a standard nick translation reaction. The clone, maize 5S rDNA (54 (link)), was labeled with biotin-16-dUTP (Roche). The BAC clones were labeled with either biotin-16-dUTP or digoxigenin-11-dUTP using a nick translation reaction. Biotin- and digoxigenin-labeled probes were detected with Alexa Fluor 488 streptavidin antibody (Invitrogen) and rhodamine-conjugated antidigoxigenin antibody (Roche), respectively.

Koo D.H., Molin W.T., Saski C.A., Jiang J., Putta K., Jugulam M., Friebe B, & Gill B.S. (2018). Extrachromosomal circular DNA-based amplification and transmission of herbicide resistance in crop weed Amaranthus palmeri. Proceedings of the National Academy of Sciences of the United States of America, 115(13), 3332-3337.

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Fluorescent DNA Probe Labeling Protocol

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The ChIPed DNAs were labeled with digoxigenin-16-dUTP using a nick translation reaction. The clone, maize 45S rDNA (Koo and Jiang 2009) was labeled with biotin-11-dUTP (Roche, Indianapolis, IN). Biotin-and digoxigenin-labeled probes were detected with Alexa Fluor 488 streptavidin antibody (Invitrogen) and rhodamine-conjugated anti-digoxigenin antibody (Roche), respectively.
Chromosomes were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) in Vectashield antifade solution (Vector Laboratories, Burlingame, CA). Images were captured with a Zeiss Axioplan 2 microscope (Carl Zeiss Microscopy LLC, Thornwood, NY) using a cooled CCD camera CoolSNAP HQ2 (Photometrics, Tucson, AZ) and AxioVision 4.8 software. The final contrast of the images was processed using Adobe Photoshop CS5 software.

Song J., Xie W., Wang S., Guo Y., Koo D., Kudrna D., Huang Y., Feng J., Zhang W., Zhou Y., Zuccolo A., Long E., Lee S., Talag J., Zhou R., Zhu X., Yuan D., Udall J.A., Xie W., Wing R.A., Zhang Q., Poland J., Zhang J., & Chen L. (2020). Assembly and Validation of Two Gap-free Reference Genomes forXian/indicaRice Reveals Insights into Plant Centromere Architecture.

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