Anti 14 3 3σ

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-14-3-3σ is a laboratory reagent used for the detection and quantification of the 14-3-3σ protein in biological samples. 14-3-3σ is a member of the 14-3-3 protein family, which play important roles in various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunoprecipitation to analyze the expression and localization of 14-3-3σ.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Anti-14-3-3 (15 citations)

2 protocols using anti 14 3 3σ

Protein Detection Using Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

To detect proteins, antibodies were used as follows: anti-RNF126 (Santa Cruz Biotechnology, Dallas TX USA, clone C-1, 1:200); anti-14–3-3σ (Santa Cruz Biotechnology, clone C-18, 1:200); anti-FLAG (Sigma-Aldrich, clone M2, 1:1000); anti-GST (Santa Cruz Biotechnology, 1–109, 1:200), anti-β-actin (Sigma-Aldrich, clone AC-74, 1:50000); anti-His (Santa Cruz Biotechnology, Clone H-15, 1:200); and anti-phospho-histone H3-Ser10 (Cell Signaling Technology, #3377S). Cell Signaling Technology’s goat anti-mouse IgG–horseradish peroxidase (HRP) (#7076S, 1:1000) and goat anti-rabbit IgG-HRP (#7074S, 1:1000) were subsequently used as secondary antibodies. The primary antibodies used for immunofluorescence were anti-CDK1 (Abcam, ab133327, 1:100), and anti-cyclin B1 (Santa Cruz Biotechnology, sc-245, 1:100). Thermo Fisher Scientific’s goat anti-mouse IgG (H+L) Alexa Fluor 594 (A-11032, 1:400), and chicken anti-rabbit IgG (H+L) Alexa Fluor 488 (A-21441, 1:400) were used as secondary antibodies.

Fa P., Qiu Z., Wang Q.E., Yan C, & Zhang J. (2021). A novel role for RNF126 in the promotion of G2 arrest via interaction with 14–3-3σ. International journal of radiation oncology, biology, physics, 112(2), 542-553.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

The expression of proteins in cells were determined using western blot analysis as previously reported [15 ]. In this study, the primary antibodies were anti-14-3-3 σ (Santa Cruz, Dallas, TX, USA), anti-β-actin (Santa Cruz), anti-ATM (Santa Cruz), anti-ATR (Santa Cruz), anti-Cdc2 (Abcam, Cambridge, MA, USA), anti-Cdc25c (Santa Cruz), anti-Chk1 (Santa Cruz), anti-Chk2 (Santa Cruz), anti-cyclin B1 (Abcam), anti-p53 (Cell Signaling Technology Inc, Danvers, MA, USA), anti-phospho-p53 Ser 15 (Cell Signaling Technology Inc), anti-phospho-ATM (Santa Cruz), anti-phospho-ATR (Santa Cruz), anti-phospho-Cdc2 (Santa Cruz), anti-phospho-Chk1 (Santa Cruz), anti-phospho-Chk2 (Santa Cruz), anti-β-tubulin (Abcam), and anti-Wee1 (Santa Cruz) antibodies. An enhanced chemiluminescence (ECL, Sigma-Aldrich) system was used for developing signals of the blots, which were analyzed using a LAS3000 system (Fujifilm, Tokyo, Japan).

Ho S.T., Lin C.C., Tung Y.T, & Wu J.H. (2019). Molecular Mechanisms Underlying Yatein-Induced Cell-Cycle Arrest and Microtubule Destabilization in Human Lung Adenocarcinoma Cells. Cancers, 11(9), 1384.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!