Magnesium xl fs

Extracellular magnesium (Mg) concentration was analyzed photometrically using a diagnostic kit (Magnesium XL FS, DiaSys Diagnostic Systems, Holzheim, Germany). The principle of this test lies in the reaction between xylidyl blue (110 µmol/L) with Mg ions in alkaline solution which results in a purple complex. Ten microliters of blank/standard/tested sample were mixed with 1000 µL of reagent. After 5 min at room temperature, the absorbance was measured at 520–628 nm by RX Monza biochemical analyzer (Randox Laboratories, Crumlin, UK). The results are defined in mM/L.

The concentrations of calcium and magnesium were measured by using a diagnostic reagent for quantitative in vitro determination produced by DiaSys Diagnostic Systems (Calcium AS FS and Magnesium XL FS, DiaSys Diagnostic Systems GmbH, Holzheim, Germany). Calcium levels were measured using the Arsenazo III test according to the manufacturer's recommendations. The test measuring range was within 0.04–20.00 mg/dL. Magnesium concentrations were measured by photometric test using xylidyl blue according to the manufacturer's procedures. The test measurements range was from 0.05 to 5.00 mg/dL, and the analyzer Konelab 20i® (ThermoScientific, Vantaa, Finland) was used for determinations.